Rabbit VDU2 / USP20 Polyclonal Antibody | anti-VDU2 antibody

Rabbit Polyclonal to Human VDU2 / USP20

Cat. Num. AAA240162

• Gene Names: USP20; VDU2; hVDU2; LSFR3A
• Reactivity: Bat, Gibbon, Bovine, Chicken, Dog, Gorilla, Horse, Human, Monkey, Orangutan, Pig, Rabbit; Predicted Reactivity: Mouse, Rat, Hamster (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: VDU2 / USP20; Polyclonal Antibody; Rabbit Polyclonal to Human VDU2 / USP20; Anti-VDU2 / USP20 Antibody (Internal) IHC-plus; USP20; Deubiquitinating enzyme 20; Ubiquitin specific protease 20; Ubiquitin thioesterase 20; HVDU2; KIAA1003; LSFR3A; Ubiquitin thiolesterase 20; VDU2; Human VDU2; anti-VDU2 antibody

• Host: Rabbit
• Reactivity: Bat, Gibbon, Bovine, Chicken, Dog, Gorilla, Horse, Human, Monkey, Orangutan, Pig, Rabbit; Predicted Reactivity: Mouse, Rat, Hamster (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human USP20. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 0.6 mg/ml (varies by lot)
• Sequence Length: 914

• Applicable Applications for anti-VDU2 antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (10 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic 15 amino acid peptide from internal region of human USP20. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Dog, Bat, Bovine, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Turkey.
• Immunogen Type: Synthetic peptide
• Immunogen: VDU2 / USP20 antibody was raised against synthetic 15 amino acid peptide from internal region of human USP20. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Dog, Bat, Bovine, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%); Mouse, Rat, Hamster (93%); Zebrafish, Stickleback (87%); Pufferfish (80%).
• Antigen Modification: Internal
• Preparation and Storage: Long term: -70 degree C; Short term: +4 degree C

Immunohistochemistry (IHC)

(Anti-USP20 antibody IHC of human adrenal medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-VDU2 antibody

VDU2 (pVHL-interacting deubiquitinating enzyme-2), is a substrate of von Hippel-Lindau tumor suppressor protein (pVHL) E3 ligase complex. VDU2 contains the signature motifs of the ubiquitin-specific processing protease family and possesses deubiquitinating activity. VDU2 and VDU1 define a subfamily of ubiquitin specific processing proteases.

Product Categories/Family for anti-VDU2 antibody

Family: Protease; Subfamily: Cysteine C19

NCBI and Uniprot Product Information

• NCBI GI #: 183397134
• NCBI GeneID: 10868
• NCBI Accession #: NP_006667.3
• NCBI GenBank Nucleotide #: NM_006676.7
• UniProt Accession #: Q9Y2K6; Q541F1; Q8IXQ1; Q96LG5; Q9UQN8; Q9UQP0
• Molecular Weight: 102,003 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 20
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 20
• NCBI Official Symbol: USP20
• NCBI Official Synonym Symbols: VDU2; hVDU2; LSFR3A
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 20; ubiquitin thioesterase 20; deubiquitinating enzyme 20; ubiquitin thiolesterase 20; ubiquitin-specific-processing protease 20; pVHL-interacting deubiquitinating enzyme 2
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 20
• UniProt Gene Name: USP20
• UniProt Synonym Gene Names: KIAA1003; LSFR3A; VDU2; hVDU2
• UniProt Entry Name: UBP20_HUMAN

NCBI Description

This gene encodes a ubiquitin specific processing protease that was first identified as a substrate of the VHL (von Hippel-Lindau disease) protein E3 ubiquitin ligase complex. In addition to being ubiquitinated by the VHL-E3 ligase complex, this enzyme deubiquitinates hypoxia-inducible factor (HIF)-1 alpha and thereby causes increased expression of HIF-1alpha targeted genes which play a role in angiogenesis, glucose metabolism, cell proliferation and metastasis. The enzyme encoded by this gene also regulates G-protein coupled receptor signaling by mediating the deubiquitination of beta-2 adrenergic receptor (ADRB2). This enzyme is a ubiquitously expressed thiolester hydrolase. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jan 2013]

Uniprot Description

USP20: Deubiquitinating enzyme involved in beta-2 adrenergic receptor (ADRB2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the peptidase C19 family. USP20/USP33 subfamily.

Protein type: Protease; Ubiquitin conjugating system; Ubiquitin-specific protease; EC 3.4.19.12

Chromosomal Location of Human Ortholog: 9q34.11

Cellular Component: centrosome; perinuclear region of cytoplasm

Molecular Function: protein binding; G-protein-coupled receptor binding; zinc ion binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; endocytosis; regulation of G-protein coupled receptor protein signaling pathway