Rabbit anti-Human, Mouse USP20 Polyclonal Antibody | anti-USP20 antibody

USP20, ID (USP20, KIAA1003, LSFR3A, VDU2, Ubiquitin carboxyl-terminal hydrolase 20, Deubiquitinating enzyme 20, Ubiquitin thioesterase 20, Ubiquitin-specific-processing protease 20, VHL-interacting deubiquitinating enzyme 2) (AP)

Cat. Num. AAA6361883

• Gene Names: USP20; VDU2; hVDU2; LSFR3A
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: USP20; Polyclonal Antibody; ID (USP20; KIAA1003; LSFR3A; VDU2; Ubiquitin carboxyl-terminal hydrolase 20; Deubiquitinating enzyme 20; Ubiquitin thioesterase 20; Ubiquitin-specific-processing protease 20; VHL-interacting deubiquitinating enzyme 2) (AP); VHL-interacting deubiquitinating enzyme 2; anti-USP20 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-USP20 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: USP20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309-339 amino acids from the Central region of human USP20.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(USP20 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the USP20 antibody detected the USP20 protein (arrow).)

Western Blot (WB)

(USP20 Antibody (Center) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the USP20 antibody detected the USP20 protein (arrow).)

Related Product Information for anti-USP20 antibody

Deubiquitinating enzyme involved in beta-2 adrenergic receptor (ADRB2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity. Mediates deubiquitination of both 'Lys-48'-and 'Lys-63'-linked polyubiquitin chains.

Product Categories/Family for anti-USP20 antibody

Antibodies; Ubiquitin

References

Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010). Oguri, M., et al. Am. J. Hypertens. 23(1):70-77(2010). Berthouze, M., et al. EMBO J. 28(12):1684-1696(2009). Olsen, J.V., et al. Cell 127(3):635-648(2006). Li, Z., et al. EMBO Rep. 6(4):373-378(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 183397134
• NCBI GeneID: 10868
• NCBI Accession #: NP_006667.3; NP_001008563.2
• NCBI GenBank Nucleotide #: NM_006676.7
• UniProt Accession #: Q9Y2K6; Q541F1; Q8IXQ1; Q96LG5; Q9UQN8; Q9UQP0
• Molecular Weight: 102,003 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 20
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 20
• NCBI Official Symbol: USP20
• NCBI Official Synonym Symbols: VDU2; hVDU2; LSFR3A
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 20; ubiquitin thioesterase 20; deubiquitinating enzyme 20; ubiquitin thiolesterase 20; ubiquitin-specific-processing protease 20; pVHL-interacting deubiquitinating enzyme 2
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 20
• UniProt Gene Name: USP20
• UniProt Synonym Gene Names: KIAA1003; LSFR3A; VDU2
• UniProt Entry Name: UBP20_HUMAN

NCBI Description

This gene encodes a ubiquitin specific processing protease that was first identified as a substrate of the VHL (von Hippel-Lindau disease) protein E3 ubiquitin ligase complex. In addition to being ubiquitinated by the VHL-E3 ligase complex, this enzyme deubiquitinates hypoxia-inducible factor (HIF)-1 alpha and thereby causes increased expression of HIF-1alpha targeted genes which play a role in angiogenesis, glucose metabolism, cell proliferation and metastasis. The enzyme encoded by this gene also regulates G-protein coupled receptor signaling by mediating the deubiquitination of beta-2 adrenergic receptor (ADRB2). This enzyme is a ubiquitously expressed thiolester hydrolase. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jan 2013]