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Western Blot (WB) (Western Blot analysis of various cells using Ribosomal Protein S27L Polyclonal Antibody)

Rabbit anti-Human, Mouse Ribosomal Protein S27L Polyclonal Antibody | anti-S27L antibody

Ribosomal Protein S27L Polyclonal Antibody

Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
Ribosomal Protein S27L; Polyclonal Antibody; Ribosomal Protein S27L Polyclonal Antibody; RPS27L; 40S ribosomal protein S27-like; anti-S27L antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
Ribosomal Protein S27L Polyclonal Antibody detects endogenous levels of Ribosomal Protein S27L protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applicable Applications for anti-S27L antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)
Application Notes
WB: 1/500-1/2000
IHC: 1/100-1/300
ELISA: 1/10,000
Immunogen
The antiserum was produced against synthesized peptide derived from human RPS27L. AA range: 1-50
Preparation and Storage
Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of various cells using Ribosomal Protein S27L Polyclonal Antibody)

Western Blot (WB) (Western Blot analysis of various cells using Ribosomal Protein S27L Polyclonal Antibody)

Western Blot (WB)

(Western blot analysis of lysates from COLO cells, using RPS27L Antibody. The lane on the right is blocked with the synthesized peptide.)

Western Blot (WB) (Western blot analysis of lysates from COLO cells, using RPS27L Antibody. The lane on the right is blocked with the synthesized peptide.)

Western Blot (WB)

(Western blot analysis of the lysates from COLO205 cells using RPS27L antibody.)

Western Blot (WB) (Western blot analysis of the lysates from COLO205 cells using RPS27L antibody.)
Related Product Information for anti-S27L antibody
Background: ribosomal protein S27 like(RPS27L) Homo sapiens. This gene encodes a protein sharing 96% amino acid similarity with ribosomal protein S27, which suggests the encoded protein may be a component of the 40S ribosomal subunit. [provided by RefSeq, Jul 2008]

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
9,477 Da
NCBI Official Full Name
40S ribosomal protein S27-like
NCBI Official Synonym Full Names
ribosomal protein S27-like
NCBI Official Symbol
RPS27L
NCBI Protein Information
40S ribosomal protein S27-like
UniProt Protein Name
40S ribosomal protein S27-like
UniProt Gene Name
RPS27L
UniProt Entry Name
RS27L_HUMAN

NCBI Description

This gene encodes a protein sharing 96% amino acid similarity with ribosomal protein S27, which suggests the encoded protein may be a component of the 40S ribosomal subunit. [provided by RefSeq, Jul 2008]

Uniprot Description

RPS27L: a protein sharing 96% amino acid similarity with ribosomal protein S27, which suggests the encoded protein may be a component of the 40S ribosomal subunit. [provided by RefSeq, Jul 2008]

Protein type: Translation; Ribosomal

Chromosomal Location of Human Ortholog: 15q22.2

Cellular Component: ribosome; nucleus

Molecular Function: protein binding; translation activator activity; structural constituent of ribosome; metal ion binding; caspase activator activity

Biological Process: G1 DNA damage checkpoint; caspase activation; positive regulation of translation; translation; DNA repair; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; response to DNA damage stimulus

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Product Notes

The S27L rps27l (Catalog #AAA3002498) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ribosomal Protein S27L Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Ribosomal Protein S27L can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA). WB: 1/500-1/2000 IHC: 1/100-1/300 ELISA: 1/10,000. Researchers should empirically determine the suitability of the S27L rps27l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ribosomal Protein S27L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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