Western Blot (WB)
((0.3ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat VDP p115 Polyclonal Antibody | anti-USO1 antibody
Goat anti-VDP p115 Antibody
Western Blot: Approx 110kDa band observed in lysates of cell line K562 (calculated MW of 108kDa according to NP_003706.1). Recommended concentration: 0.3-1ug/ml.
Western Blot (WB)
((0.3ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
Uniprot Description
TAP: General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity. Belongs to the VDP/USO1/EDE1 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Vesicle; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 4q21.1
Cellular Component: cytosol; endoplasmic reticulum; Golgi apparatus; Golgi membrane; Golgi stack; membrane; nucleolus; perinuclear region of cytoplasm; transport vesicle
Molecular Function: protein binding; protein transporter activity
Biological Process: COPII coating of Golgi vesicle; ER to Golgi vesicle-mediated transport; Golgi vesicle docking; intracellular protein transport; transcytosis; vesicle fusion with Golgi apparatus
Research Articles on USO1
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Product Notes
The USO1 uso1 (Catalog #AAA420500) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-VDP p115 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's VDP p115 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 2000. Western Blot: Approx 110kDa band observed in lysates of cell line K562 (calculated MW of 108kDa according to NP_003706.1). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the USO1 uso1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DESEDPGKDL DH. It is sometimes possible for the material contained within the vial of "VDP p115, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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