Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Soluble protein-100 (S-100) Standard Curve.)
Mouse Soluble Protein-100 (S-100) ELISA Kit | S-100 elisa kit
Mouse Soluble Protein-100 (S-100) ELISA Kit
Gene Names
S100a1; S100; S100a; AI266795
Reactivity
Mouse
Synonyms
Soluble Protein-100 (S-100); Mouse Soluble Protein-100 (S-100) ELISA Kit; S100A1; S100; S100-alpha; S100A; S100 alpha; S100 calcium-binding protein A1; S100 protein; alpha polypeptide; S-100 elisa kit
Reactivity
Mouse
Specificity
Mouse Soluble protein-100 (S-100) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse S100A1. No significant cross-reactivity or interference between Mouse S100A1 and analogues was observed.
Sequence Length
94
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Soluble protein-100 (S-100) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Soluble protein-100 (S-100) Standard Curve.)
Related Product Information for S-100 elisa kit
Description: This Mouse Soluble protein-100 (S-100) ELISA Kit employs a two-site sandwich ELISA to quantitate S100A1 in samples. An antibody specific for S100A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any S100A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: A1is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. S100A1 overexpression enhances cardiac contractile performance which suggests that S100A1 is a regulator of myocardial contractility. S100A1 improves cardiac performance both by regulating calcium ion handling by the sarcoplasmic reticulum and the responsiveness of myofibrils to calcium ion.
Background: A1is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. S100A1 overexpression enhances cardiac contractile performance which suggests that S100A1 is a regulator of myocardial contractility. S100A1 improves cardiac performance both by regulating calcium ion handling by the sarcoplasmic reticulum and the responsiveness of myofibrils to calcium ion.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein S100-A1
NCBI Official Synonym Full Names
S100 calcium binding protein A1
NCBI Official Symbol
S100a1
NCBI Official Synonym Symbols
S100; S100a; AI266795
NCBI Protein Information
protein S100-A1
UniProt Protein Name
Protein S100-A1
UniProt Gene Name
S100a1
UniProt Entry Name
S10A1_MOUSE
Uniprot Description
S100A1: Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. Interacts with AGER and CAPZA1. Interacts with FKBP4. Interacts with RYR1 and RYR2. Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain. Belongs to the S-100 family.
Protein type: Calcium-binding; Endoplasmic reticulum
Cellular Component: I band; neuron projection; sarcoplasmic reticulum; cytoplasm; M band; nucleus; Z disc; A band
Molecular Function: identical protein binding; protein binding; protein homodimerization activity; metal ion binding; calcium ion binding; calcium-dependent protein binding; ATPase binding
Biological Process: negative regulation of transcription from RNA polymerase II promoter; regulation of heart contraction
Research Articles on S-100
Similar Products
Product Notes
The Mouse S-100 s100a1 (Catalog #AAA9717101) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717101 ELISA Kit recognizes Mouse S-100. It is sometimes possible for the material contained within the vial of "Soluble Protein-100 (S-100), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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