Mouse anti-Human US01 / p115 Monoclonal Antibody | anti-US01 antibody

Mouse Monoclonal [clone 3F4] (IgG1,k) to Human US01 / p115

Cat. Num. AAA249040

• Gene Names: USO1; TAP; VDP; P115
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: US01 / p115; Monoclonal Antibody; Mouse Monoclonal [clone 3F4] (IgG1; k) to Human US01 / p115; Anti-US01 / p115 Antibody (clone 3F4) IHC-plus; USO1; p115; Protein USO1 homolog; TAP; Vesicle docking protein p115; Vesicle-docking protein; US01; USO1 vesicle transport factor; VDP; Human US01; anti-US01 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3F4
• Specificity: Human USO1
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 962

• Applicable Applications for anti-US01 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB; Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using cell line lysates and the recombinant protein used as the immunogen.
• Target Species: Human
• Immunogen Description: USO1 (AAH32654, 1 a.a. ~ 962 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: US01 / p115 antibody was raised against uSO1 (AAH32654, 1 a.a. ~ 962 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C or lower. Aliquot to avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-USO1 / p115 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-USO1 / p115 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Antibody. Western blot of USO1 expression in HeLa.)

ELISA (EIA)

(Detection limit for recombinant GST tagged USO1 is 0.3 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 4505541
• NCBI GeneID: 8615
• NCBI Accession #: NP_003706.1
• NCBI GenBank Nucleotide #: NM_003715.2
• UniProt Accession #: O60763; Q6PK63; Q86TB8; Q8N592; B2RAQ0
• Molecular Weight: 109,195 Da
• NCBI Official Full Name: general vesicular transport factor p115
• NCBI Official Synonym Full Names: USO1 vesicle transport factor
• NCBI Official Symbol: USO1
• NCBI Official Synonym Symbols: TAP; VDP; P115
• NCBI Protein Information: general vesicular transport factor p115; vesicle docking protein p115; transcytosis associated protein; USO1 vesicle docking protein homolog
• UniProt Protein Name: General vesicular transport factor p115
• UniProt Gene Name: USO1
• UniProt Synonym Gene Names: VDP; TAP
• UniProt Entry Name: USO1_HUMAN

NCBI Description

The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Uniprot Description

TAP: General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity. Belongs to the VDP/USO1/EDE1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q21.1

Cellular Component: Golgi membrane; Golgi apparatus; Golgi stack; membrane; endoplasmic reticulum; perinuclear region of cytoplasm; nucleolus; cytosol

Molecular Function: protein transporter activity

Biological Process: intracellular protein transport; ER to Golgi vesicle-mediated transport; Golgi vesicle docking; vesicle fusion with Golgi apparatus; transcytosis; mitotic cell cycle