Rabbit Vav Polyclonal Antibody | anti-VAV1 antibody

Vav, phosphorylated (Tyr174) (Proto-oncogene VAV, VAV1)

Cat. Num. AAA631319

• Gene Names: VAV1; VAV
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Vav; Polyclonal Antibody; phosphorylated (Tyr174) (Proto-oncogene VAV; VAV1); Anti -Vav; anti-VAV1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Vav when phosphorylated (Tyr174). Species Cross-Reactivity: mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-VAV1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot:1:500-1:1,000
• Immunogen: Synthetic phosphopeptide corresponding to human Vav around the phosphorylation site of tyrosine 174 (E-I-YP-E-D).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-VAV1 antibody

The Vav family are Rho/Rac guanosine nucleotide exchange factors (GEFs), consisting of three members in mammalian cells (Vav, Vav2, Vav3) and one in nematodes (CelVav). First discovered based on its transforming properties, Vav is expressed mainly in hematopoietic cells and a few non-hematopoietic tissues, such as the pancreas and tooth enamels. As a signaling transducer, Vav is involved in T-cell activated transduction of T-cell antigen receptor (TCR). T-cell stimulated and tyrosine phosphorylated Vav acts as a catalyst in the exchange of guanosine nucleotides on Rac-1, a GTP binding protein. Using a mouse model, Vav expression has been determined to play an essential role in the cyctosketetal, proliferative, and apoptotic pathways for developing lymphoid cells and its signal response.

Product Categories/Family for anti-VAV1 antibody

Antibodies; Abs to GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 37645
• NCBI GeneID: 7409
• UniProt Accession #: P15498; Q15860; Q96D37
• Molecular Weight: 98,314 Da
• NCBI Official Full Name: VAV
• NCBI Official Synonym Full Names: vav 1 guanine nucleotide exchange factor
• NCBI Official Symbol: VAV1
• NCBI Official Synonym Symbols: VAV
• NCBI Protein Information: proto-oncogene vav; vav 1 oncogene; vav proto-oncogene
• UniProt Protein Name: Proto-oncogene vav
• Protein Family: Proto-oncogene
• UniProt Gene Name: VAV1
• UniProt Synonym Gene Names: VAV
• UniProt Entry Name: VAV_HUMAN

NCBI Description

The protein encoded by this proto-oncogene is a member of the Dbl family of guanine nucleotide exchange factors (GEF) for the Rho family of GTP binding proteins. The protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. This particular GEF has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. [provided by RefSeq]

Uniprot Description

VAV1: a proto-oncogenic guanine nucleotide exchange factors (GEF) of the Dbl family. A GEF for the Rho family of GTP binding proteins. Expressed exclusively in hematopoietic cells. Important in hematopoiesis, playing a role in T-cell and B-cell development and activation.

Protein type: Motility/polarity/chemotaxis; GEFs, Rac/Rho; Oncoprotein; GEFs

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: plasma membrane; intercellular junction; cytosol

Molecular Function: protein binding; guanyl-nucleotide exchange factor activity; metal ion binding; Rac guanyl-nucleotide exchange factor activity; transcription factor activity

Biological Process: epidermal growth factor receptor signaling pathway; integrin-mediated signaling pathway; neutrophil chemotaxis; platelet activation; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; T cell activation; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; G-protein coupled receptor protein signaling pathway; regulation of small GTPase mediated signal transduction; regulation of transcription, DNA-dependent; small GTPase mediated signal transduction; T cell costimulation; innate immune response; blood coagulation; vascular endothelial growth factor receptor signaling pathway