Rabbit anti-Human VAV1 Polyclonal Antibody | anti-VAV1 antibody

VAV1, ID (VAV1, VAV, Proto-oncogene vav) (Azide free) (HRP)

Cat. Num. AAA6362338

• Gene Names: VAV1; VAV
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: VAV1; Polyclonal Antibody; ID (VAV1; VAV; Proto-oncogene vav) (Azide free) (HRP); Proto-oncogene vav; anti-VAV1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-VAV1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: VAV1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 436-466 amino acids from the Central region of human VAV1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(VAV1 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the VAV1 antibody detected the VAV1 protein (arrow).)

Related Product Information for anti-VAV1 antibody

The protein encoded by this proto-oncogene is a member of the Dbl family of guanine nucleotide exchange factors (GEF) for the Rho family of GTP binding proteins. The protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. This particular GEF has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication.

Product Categories/Family for anti-VAV1 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Hollmann, A., et al. Hematol Oncol 28(3):142-150(2010). Barda-Saad, M., et al. EMBO J. 29(14):2315-2328(2010). Davila, S., et al. Genes Immun. 11(3):232-238(2010). Kim, H.S., et al. Immunity 32(2):175-186(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 7108367
• NCBI GeneID: 7409
• NCBI Accession #: NP_005419.2
• NCBI GenBank Nucleotide #: NM_005428.3
• UniProt Accession #: P15498; Q15860
• Molecular Weight: 98,314 Da
• NCBI Official Full Name: proto-oncogene vav isoform 1
• NCBI Official Synonym Full Names: vav 1 guanine nucleotide exchange factor
• NCBI Official Symbol: VAV1
• NCBI Official Synonym Symbols: VAV
• NCBI Protein Information: proto-oncogene vav; vav 1 oncogene
• UniProt Protein Name: Proto-oncogene vav
• Protein Family: Proto-oncogene
• UniProt Gene Name: VAV1
• UniProt Synonym Gene Names: VAV
• UniProt Entry Name: VAV_HUMAN

NCBI Description

This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. The encoded protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. The encoded protein has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

Function: Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.

Subunit structure: May interact with CCPG1

By similarity. Interacts with APS, DOCK2, GRB2, GRB3, DOCK2, SLA, TEC and ZNF655/VIK. Interacts with SIAH2; without leading to its degradation. Associates with BLNK, PLCG1, GRB2 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with CBLB; which inhibits tyrosine phosphorylation and down-regulates activity. Interacts with SHB and CLNK

By similarity. Interacts with THEMIS2. Interacts with HCK

By similarity. Interacts with NEK3 and this interaction is prolactin-dependent. Interacts with ITK. Interacts with PTK2B/PYK2. Interacts (via SH2 domain) with SYK. Interacts with ANKRD54

By similarity. Ref.8 Ref.9 Ref.10 Ref.11 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.19 Ref.20

Tissue specificity: Widely expressed in hematopoietic cells but not in other cell types.

Domain: The DH domain is involved in interaction with CCPG1

By similarity.

Post-translational modification: Phosphorylated on tyrosine residues by HCK in response to IFNG and bacterial lipopolysaccharide (LPS)

By similarity. Phosphorylated by FYN. Ref.12

Miscellaneous: 'Vav' stands for the sixth letter of the Hebrew alphabet.

Sequence similarities: Contains 1 CH (calponin-homology) domain.Contains 1 DH (DBL-homology) domain.Contains 1 PH domain.Contains 1 phorbol-ester/DAG-type zinc finger.Contains 1 SH2 domain.Contains 2 SH3 domains.

Sequence caution: The sequence CAA34383.1 differs from that shown. Reason: Frameshift at positions 322 and 355.