Rabbit anti-Human CEP55 Polyclonal Antibody | anti-CEP55 antibody

CEP55 Antibody

Cat. Num. AAA9605374

• Gene Names: CEP55; CT111; MARCH; URCC6; C10orf3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: CEP55; Polyclonal Antibody; CEP55 Antibody; C10orf3; cancer/testis antigen 111; Centrosomal protein 55kDa; Centrosomal protein of 55 kDa; CEP 55; Cep55; CEP55_HUMAN; CT111; FLJ10540; Up regulated in colon cancer 6; Up-regulated in colon cancer 6; URCC 6; URCC6; anti-CEP55 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CEP55 antibody detects endogenous levels of total CEP55
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 464

• Applicable Applications for anti-CEP55 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthesized peptide
• Subcellular Location: Cytoplasm > Cytoskeleton > Centrosome > Centriole. Cytoplasm > Cytoskeleton > Centrosome. Cleavage Furrow. Midbody. Present at the centrosomes at interphase. A small portion is associated preferentially with the mother centriole, whereas the majority localizes to the pericentriolar material. During mitosis, loss of affinity for the centrosome at the onset of prophase and diffusion throughout the cell. This dissociation from the centrosome is phosphorylation-dependent. May remain localized at the centrosome during mitosis in certain cell types. Appears at the cleavage furrow in late anaphase and in the midbody in cytokinesis.
• Tissue Specificity: Expressed in embryonic brain (PubMed:28264986). Expressed in fetal brain ganglionic eminence, kidney tubules and multinucleate neurons in the temporal cortex (PubMed:28264986). Expressed in adult brain, cerebellum, kidney tubules, intestine and muscles (at protein level) (PubMed:28295209, PubMed:28264986). Widely expressed, mostly in proliferative tissues. Highly expressed in testis. Intermediate levels in adult and fetal thymus, as well as in various cancer cell lines. Low levels in different parts of the digestive tract, bone marrow, lymph nodes, placenta, fetal heart and fetal spleen. Hardly detected in brain.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from 293, using CEP55 Antibody. Lane 1 was treated with the antigen-specific peptide.)

Western Blot (WB)

(Western blot analysis of extracts from HUVEC cells using CEP55 antibody.The lane on the left is treated with the antigen-specific peptide.)

Related Product Information for anti-CEP55 antibody

Function: Plays a role in mitotic exit and cytokinesis (PubMed:16198290, PubMed:17853893). Recruits PDCD6IP and TSG101 to midbody during cytokinesis. Required for successful completion of cytokinesis (PubMed:17853893). Not required for microtubule nucleation (PubMed:16198290). Plays a role in the development of the brain and kidney (PubMed:28264986).
Subunit Structure: Homodimer (PubMed:16406728). Interacts (phosphorylated on Ser-425 and Ser-428) with PLK1 (PubMed:16198290). Interacts with AKAP9/CG-NAP; the interaction occurs in interphase and is lost upon mitotic entry (PubMed:16198290). Interacts with PCNT/Kendrin; the interaction occurs in interphase and is lost upon mitotic entry (PubMed:16198290). Directly interacts with PDCD6IP; this interaction is required for PDCD6IP targeting to the midbody; CEP55 binds PDCD6IP in a 2:1 stoichiometry; PDCD6IP competes with TSG101 for the same binding site (PubMed:17853893, PubMed:17556548, PubMed:18641129, PubMed:18948538). Interacts with TSG101; TSG101 competes with PDCD6IP for the same binding site; interaction is required for cytokinesis but not for viral budding (PubMed:17853893, PubMed:17556548, PubMed:18948538). Interacts with MVB12A, VPS37B, VPS37C and VPS28 (PubMed:17853893).
Post-translational Modifications: There is a hierachy of phosphorylation, where both Ser-425 and Ser-428 are phosphorylated at the onset of mitosis, prior to Ser-436. Phosphorylation at Ser-425 and Ser-428 is required for dissociation from the centrosome at the G2/M boundary. Phosphorylation at the 3 sites, Ser-425, Ser-428 and Ser-436, is required for protein function at the final stages of cell division to complete cytokinesis successfully.

NCBI and Uniprot Product Information

• NCBI GI #: 187608545
• NCBI GeneID: 55165
• NCBI Accession #: NP_001120654.1
• NCBI GenBank Nucleotide #: NM_001127182.1
• UniProt Accession #: Q53EZ4; Q32WF5; Q3MV20; Q5VY28; Q6N034; Q96H32; Q9NVS7; B2RDG8; D3DR37
• Molecular Weight: Observed: 54 kD; Predicted: 55 kDa
• NCBI Official Full Name: centrosomal protein of 55 kDa
• NCBI Official Synonym Full Names: centrosomal protein 55
• NCBI Official Symbol: CEP55
• NCBI Official Synonym Symbols: CT111; MARCH; URCC6; C10orf3
• NCBI Protein Information: centrosomal protein of 55 kDa
• UniProt Protein Name: Centrosomal protein of 55 kDa
• Protein Family: Centrosomal protein
• UniProt Gene Name: CEP55
• UniProt Synonym Gene Names: C10orf3ImportedManual assertion inferred from database entriesiHGNC:1161; URCC6; Cep55

Uniprot Description

Plays a role in mitotic exit and cytokinesis (PubMed:16198290, PubMed:17853893). Recruits PDCD6IP and TSG101 to midbody during cytokinesis. Required for successful completion of cytokinesis (PubMed:17853893). Not required for microtubule nucleation (PubMed:16198290). Plays a role in the development of the brain and kidney (PubMed:28264986).