Rabbit anti-Human, Mouse Vascular Endothelial Growth Factor Receptor 2 Polyclonal Antibody | anti-KDR antibody

Vascular Endothelial Growth Factor Receptor 2, phosphorylated (Tyr1054, Tyr1059) (VEGF R2, Flk1, KDR, Receptor Tyrosine Kinase)

Cat. Num. AAA630303

• Gene Names: KDR; FLK1; CD309; VEGFR; VEGFR2
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography
• Synonyms: Vascular Endothelial Growth Factor Receptor 2; Polyclonal Antibody; phosphorylated (Tyr1054; Tyr1059) (VEGF R2; Flk1; KDR; Receptor Tyrosine Kinase); Anti -Vascular Endothelial Growth Factor Receptor 2; anti-KDR antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human VEGFR2 when phosphorylated at Tyr1054 and Tyr1059. Sequence Homology (100%)
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography
• Form/Format: Supplied as a liquid in Dulbecco PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG and Protease free), 50% glycerol, pH 7.3, 0.05% sodium azide.

• Applicable Applications for anti-KDR antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western blot.; Dilution: Western Blot: 1:1000; Optimal dilution to be determined by researcher.
• Immunogen: Synthetic phosphopeptide corresponding to a region of human Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) that contains Tyr1054 and Tyr1059. Species Sequence Homology: Mouse, rat
• Positive Control: Porcine aortic endothelial (PAE) cells transfected with a chimeric receptor consisting of the extracellular portion of the CSF-1 receptor fused with the transmembrane and intracellular domains of mouse VEGFR2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-KDR antibody

Vascular Endothelial Growth Factor Receptor 2 (VEGFR2, also known as KDR/FLK-1) is a ~200kD member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors upon ligand binding, VEGFR2 forms a dimer and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2- and phosphotyrosine binding domain containing signaling proteins. Phosphorylation of tyrosines 1054 and 1059 in the activation loop is required for activation of VEGFR2 and its intrinsic tyrosine kinase activity.

Product Categories/Family for anti-KDR antibody

Antibodies; Abs to Receptors; Tyrosine Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 11321597
• NCBI GeneID: 3791
• NCBI Accession #: NP_002244.1
• NCBI GenBank Nucleotide #: NM_002253.2
• UniProt Accession #: P35968; O60723; Q14178; Q59EB0
• Molecular Weight: 151,527 Da
• NCBI Official Full Name: vascular endothelial growth factor receptor 2
• NCBI Official Synonym Full Names: kinase insert domain receptor (a type III receptor tyrosine kinase)
• NCBI Official Symbol: KDR
• NCBI Official Synonym Symbols: FLK1; CD309; VEGFR; VEGFR2
• NCBI Protein Information: vascular endothelial growth factor receptor 2; soluble VEGFR2; OTTHUMP00000158928; fetal liver kinase 1; fetal liver kinase-1; protein-tyrosine kinase receptor Flk-1; tyrosine kinase growth factor receptor
• UniProt Protein Name: Vascular endothelial growth factor receptor 2
• Protein Family: Vascular endothelial growth factor receptor
• UniProt Gene Name: KDR
• UniProt Synonym Gene Names: FLK1
• UniProt Entry Name: VGFR2_HUMAN

NCBI Description

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq]

Uniprot Description

VEGFR2: a receptor tyrosine kinase of the VEGFR family. High affinity receptor for VEGF and VEGF-C. Ligand binding induces autophosphorylation and activation. Activated receptor recruits proteins including Shc, GRB2, PI3K, Nck, SHP-1 and SHP-2. Plays a key role in vascular development and regulation of vascular permeability.

Protein type: EC 2.7.10.1; Membrane protein, integral; Protein kinase, TK; Kinase, protein; Protein kinase, tyrosine (receptor); TK group; VEGFR family

Chromosomal Location of Human Ortholog: 4q11-q12

Cellular Component: Golgi apparatus; integral to plasma membrane; endoplasmic reticulum; early endosome; cytoplasmic membrane-bound vesicle; plasma membrane; extracellular region; cell junction; nucleus; endosome; lipid raft; external side of plasma membrane

Molecular Function: integrin binding; vascular endothelial growth factor receptor activity; protein binding; growth factor binding; protein-tyrosine kinase activity; Hsp90 protein binding; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: extracellular matrix organization and biogenesis; positive regulation of positive chemotaxis; peptidyl-tyrosine phosphorylation; viral reproduction; protein amino acid autophosphorylation; cell maturation; calcium ion homeostasis; regulation of cell shape; positive regulation of MAPKKK cascade; positive regulation of focal adhesion formation; ovarian follicle development; positive regulation of mesenchymal cell proliferation; positive regulation of cell proliferation; angiogenesis; vasculogenesis; endothelial cell differentiation; cell fate commitment; embryonic hemopoiesis; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of angiogenesis; branching morphogenesis of a tube; cell migration during sprouting angiogenesis; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; lymph vessel development; alveolus development; surfactant homeostasis; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis; positive regulation of cell migration; positive regulation of nitric-oxide synthase biosynthetic process

Disease: Hemangioma, Capillary Infantile