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Western Blot (WB) (Western Blot: Sample: Mouse Kidney Tissue.)

Rabbit anti-Mouse Vascular Cell Adhesion Molecule 1 (VCAM1) Polyclonal Antibody | anti-VCAM1 antibody

Polyclonal Antibody to Vascular Cell Adhesion Molecule 1 (VCAM1)

Gene Names
Vcam1; CD106; Vcam-1
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Vascular Cell Adhesion Molecule 1 (VCAM1); Polyclonal Antibody; Polyclonal Antibody to Vascular Cell Adhesion Molecule 1 (VCAM1); anti-VCAM1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against VCAM1. It has been selected for its ability to recognize VCAM1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF- PKETTIWVSP SPILEEGSPV NLTCSSDGIP APKILWSRQL NNGELQPLSE NTTLTFMSTK RDDSGIYVCE GINEAGISRK SVELIIQVSP KDIQLTVFPS KSVKEGDTVI ISCTCGNVPE TWIILKKKAK TGDMVLKSVD GSYTIRQAQL QDAGIYECES KTEVGSQLRS LT
Sequence Length
647
Applicable Applications for anti-VCAM1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant VCAM1 (Pro511~Thr682) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2043777
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Mouse Kidney Tissue.)

Western Blot (WB) (Western Blot: Sample: Mouse Kidney Tissue.)

Western Blot (WB)

(Western Blot: Sample: Recombinant VCAM1, Mouse.)

Western Blot (WB) (Western Blot: Sample: Recombinant VCAM1, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P. Samples: Mouse Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P. Samples: Mouse Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
38,372 Da
NCBI Official Full Name
Vascular cell adhesion protein 1
NCBI Official Synonym Full Names
vascular cell adhesion molecule 1
NCBI Official Symbol
Vcam1
NCBI Official Synonym Symbols
CD106; Vcam-1
NCBI Protein Information
vascular cell adhesion protein 1
UniProt Protein Name
Vascular cell adhesion protein 1
UniProt Gene Name
Vcam1
UniProt Synonym Gene Names
Vcam-1; V-CAM 1; VCAM-1

Uniprot Description

VCAM1: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3 G1|3 50.17 cM

Cellular Component: apical part of cell; cell surface; early endosome; endoplasmic reticulum; external side of plasma membrane; extracellular space; filopodium; Golgi apparatus; integral component of membrane; membrane; microvillus; plasma membrane; podosome; sarcolemma

Molecular Function: amine oxidase activity; cell adhesion molecule binding; integrin binding

Biological Process: amine metabolic process; cell adhesion; cell-matrix adhesion; heart development; heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules; leukocyte adhesion; leukocyte tethering or rolling; positive regulation of T cell proliferation; viral process

Research Articles on VCAM1

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Product Notes

The VCAM1 vcam1 (Catalog #AAA2004950) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Vascular Cell Adhesion Molecule 1 (VCAM1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Vascular Cell Adhesion Molecule 1 (VCAM1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the VCAM1 vcam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- PKETTIWVSP SPILEEGSPV NLTCSSDGIP APKILWSRQL NNGELQPLSE NTTLTFMSTK RDDSGIYVCE GINEAGISRK SVELIIQVSP KDIQLTVFPS KSVKEGDTVI ISCTCGNVPE TWIILKKKAK TGDMVLKSVD GSYTIRQAQL QDAGIYECES KTEVGSQLRS LT. It is sometimes possible for the material contained within the vial of "Vascular Cell Adhesion Molecule 1 (VCAM1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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