Vascular Cell Adhesion Molecule 1 (VCAM1) Recombinant Protein | VCAM1 recombinant protein

Recombinant Vascular Cell Adhesion Molecule 1 (VCAM1)

Cat. Num. AAA2010240

• Gene Names: Vcam1; CD106; Vcam-1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Vascular Cell Adhesion Molecule 1 (VCAM1); Recombinant Vascular Cell Adhesion Molecule 1 (VCAM1); VCAM1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-PKETTIWVSP SPILEEGSPV NLTCSSDGIP APKILWSRQL NNGELQPLSE NTTLTFMSTK RDDSGIYVCE GINEAGISRK SVELIIQVSP KDIQLTVFPS KSVKEGDTVI ISCTCGNVPE TWIILKKKAK TGDMVLKSVD GSYTIRQAQL QDAGIYECES KTEVGSQLRS LT
• Sequence Length: 739

• Applicable Applications for VCAM1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Pro511~Thr682 (Accession # P29533) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 170295823
• NCBI GeneID: 22329
• NCBI Accession #: NP_035823.3
• NCBI GenBank Nucleotide #: NM_011693.3
• UniProt Accession #: P29533
• Molecular Weight: 20.3kDa
• NCBI Official Full Name: vascular cell adhesion protein 1
• NCBI Official Synonym Full Names: vascular cell adhesion molecule 1
• NCBI Official Symbol: Vcam1
• NCBI Official Synonym Symbols: CD106; Vcam-1
• NCBI Protein Information: vascular cell adhesion protein 1; V-CAM 1
• UniProt Protein Name: Vascular cell adhesion protein 1
• Protein Family: Vascular cell adhesion protein
• UniProt Gene Name: Vcam1
• UniProt Synonym Gene Names: Vcam-1; V-CAM 1; VCAM-1
• UniProt Entry Name: VCAM1_MOUSE

Uniprot Description

VCAM1: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis

Cellular Component: Golgi apparatus; extracellular space; microvillus; cell surface; endoplasmic reticulum; early endosome; integral to membrane; membrane; apical part of cell; plasma membrane; podosome; sarcolemma; external side of plasma membrane; filopodium

Molecular Function: integrin binding; amine oxidase activity; cell adhesion molecule binding

Biological Process: heterophilic cell adhesion; cell-cell adhesion; leukocyte adhesion; viral reproduction; heart development; cell-matrix adhesion; positive regulation of T cell proliferation; amine metabolic process; cell adhesion; leukocyte tethering or rolling