Rabbit VAMP3 Polyclonal Antibody | anti-VAMP3 antibody

VAMP3, ID (VAMP3, SYB3, Vesicle-associated membrane protein 3, Cellubrevin, Synaptobrevin-3) (FITC)

Cat. Num. AAA6362183

• Gene Names: VAMP3; CEB
• Reactivity: Human, Mouse, Zebrafish
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: VAMP3; Polyclonal Antibody; ID (VAMP3; SYB3; Vesicle-associated membrane protein 3; Cellubrevin; Synaptobrevin-3) (FITC); Synaptobrevin-3; anti-VAMP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Zebrafish
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human VAMP3. Species Crossreactivity: mouse and zebrafish
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-VAMP3 antibody: Western Blot (WB), Immunohistochemistry (IHC), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 15-43 in the central region of human VAMP3, UniProt Accession #Q15836. Species sequence homology: bovine, rat and monkey
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse brain tissue lysates (35ug/lane) usingMBS6006975. This demonstrates the VAMP3 antibody detected the VAMP3 protein (arrow).)

Western Blot (WB)

(Western Blot analysis in zebra fish brain tissue lysates (35ug/lane) usingMBS6006975. This demonstrates the VAMP3 antibody detected the VAMP3 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue using MBS6006975 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Related Product Information for anti-VAMP3 antibody

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Because of its high homology to other known VAMPs, its broad tissue distribution, and its subcellular localization, the protein encoded by this gene was shown to be the human equivalent of the rodent cellubrevin. In platelets the protein resides on a compartment that is not mobilized to the plasma membrane on calcium or thrombin stimulation.

Product Categories/Family for anti-VAMP3 antibody

Antibodies; Membrane Proteins

References

1. Ban, H.J., et al. BMC Genet. 11, 26 (2010). 2. Fader, C.M., et al. Biochim. Biophys. Acta 1793(12):1901-1916(2009). 3. Kean, M.J., et al. J. Cell. Sci. 122 (PT 22), 4089-4098 (2009). 4. Vassilopoulos, S., et al. Science 324(5931):1192-1196(2009). 5. Luftman, K., et al. Biochem. Biophys. Res. Commun. 380(1):65-70(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4759300
• NCBI GeneID: 9341
• NCBI Accession #: NP_004772.1
• NCBI GenBank Nucleotide #: NM_004781.3
• UniProt Accession #: Q15836; Q9BRV4
• Molecular Weight: 11,309 Da
• NCBI Official Full Name: vesicle-associated membrane protein 3
• NCBI Official Synonym Full Names: vesicle-associated membrane protein 3
• NCBI Official Symbol: VAMP3
• NCBI Official Synonym Symbols: CEB
• NCBI Protein Information: vesicle-associated membrane protein 3; VAMP-3; cellubrevin; synaptobrevin-3
• UniProt Protein Name: Vesicle-associated membrane protein 3
• Protein Family: Vesicle-associated membrane protein
• UniProt Gene Name: VAMP3
• UniProt Synonym Gene Names: SYB3; VAMP-3; CEB
• UniProt Entry Name: VAMP3_HUMAN

NCBI Description

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Because of its high homology to other known VAMPs, its broad tissue distribution, and its subcellular localization, the protein encoded by this gene was shown to be the human equivalent of the rodent cellubrevin. In platelets the protein resides on a compartment that is not mobilized to the plasma membrane on calcium or thrombin stimulation. [provided by RefSeq, Jul 2008]

Uniprot Description

VAMP3: SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network. Belongs to the synaptobrevin family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p36.23

Cellular Component: neuron projection; cell surface; intracellular membrane-bound organelle; clathrin-coated vesicle; integral to membrane; clathrin coated vesicle membrane; cytosol; secretory granule; SNARE complex; synaptic vesicle; recycling endosome; apical plasma membrane; plasma membrane; intracellular; cell junction

Molecular Function: SNAP receptor activity; SNARE binding; protein binding; syntaxin-1 binding

Biological Process: vesicle fusion; vesicle-mediated transport; exocytosis; neurotransmitter secretion; Golgi to plasma membrane protein transport; protein complex assembly; positive regulation of immunoglobulin secretion; positive regulation of receptor recycling; retrograde transport, endosome to Golgi; vesicle docking during exocytosis; calcium ion-dependent exocytosis