Immunohistochemistry (IHC)
(Anti-TG / Thyroglobulin antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Mouse TG / Thyroglobulin Monoclonal Antibody | anti-TG antibody
Mouse Monoclonal [clone 2H11] (IgG1) to Human TG / Thyroglobulin
Gene Names
TG; TGN; AITD3
Reactivity
Dog, Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot, Immunoprecipitation, ELISA
Purity
Protein A Purified
Synonyms
TG / Thyroglobulin; Monoclonal Antibody; Mouse Monoclonal [clone 2H11] (IgG1) to Human TG / Thyroglobulin; Anti-TG / Thyroglobulin Antibody (clone 2H11) IHC-plus; TG; AITD3; TDH3; Thyroglobulin; TGN; Human TG; anti-TG antibody
Host
Mouse
Reactivity
Dog, Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2H11
Specificity
Human thyroglobulin protein. Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse myeloma SP2/0 cells after immunization with thyroglobulin from human thyroid gland using conventional hybridoma technology.
Purity/Purification
Protein A Purified
Form/Format
0.02 M potassium phosphate, 0.5 M sodium chloride, pH 7.2, 0.01% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
2768
Applicable Applications for anti-TG antibody
Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA)
Application Notes
ELISA, IHC-Fr (1:50 - 1:200), IHC-P (5 ug/ml), IP, WB (1:400)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
Target Species
Human
Immunogen Description
Fusion protein from human TG / Thyroglobulin.
Immunogen
TG / Thyroglobulin antibody was raised against fusion protein from human TG / Thyroglobulin.
Preparation and Storage
+4 degree C or -20 degree C, Avoid repeated freezing and thawing.
Immunohistochemistry (IHC)
(Anti-TG / Thyroglobulin antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC)
(Anti-TG / Thyroglobulin antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Western Blot (WB)
(Anti-Thyroglobulin Antibody - Western Blot. Western blot of Mouse Mab-anti-Thyroglobulin antibody. Separation was achieved under reducing conditions using a pre-cast 5% Tris-HCl gel from Bio-Rad Laboratories. This antibody recognizes a single 330 kD band corresponding to human thyroglobulin (left lane 3 ug, right lane 3.0 ug) as confirmed by the position of molecular weight markers (not shown). A 1:400 dilution of Mab anti-Thyroglobulin was used for 2h followed by detection using a 1:5000 dilution of IRDyeTM800 conjugated Goat-a-Mouse IgG [H&L] (and visualization using the Odyssey Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.)
Western Blot (WB)
(Anti-Thyroglobulin Antibody - Western Blot. Western blot of Mouse Mab-anti-Thyroglobulin antibody. Separation was achieved under reducing conditions using a pre-cast 5% Tris-HCl gel from Bio-Rad Laboratories. This antibody recognizes a single 330 kD band corresponding to human thyroglobulin (left lane 3 ug, right lane 3.0 ug) as confirmed by the position of molecular weight markers (not shown). A 1:400 dilution of Mab anti-Thyroglobulin was used for 2h followed by detection using a 1:5000 dilution of IRDyeTM800 conjugated Goat-a-Mouse IgG [H&L] (and visualization using the Odyssey Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.)
Western Blot (WB)
(Western blot of anti-Thyroglobulin antibody at a dilution of 1:400. This antibody recognizes a single 330 kDa band corresponding to human thyroglobulin (left lane 3 mg, right lane 3.0 mg) as confirmed by the position of molecular weight markers (not shown).)
Western Blot (WB)
(Western blot of anti-Thyroglobulin antibody at a dilution of 1:400. This antibody recognizes a single 330 kDa band corresponding to human thyroglobulin (left lane 3 mg, right lane 3.0 mg) as confirmed by the position of molecular weight markers (not shown).)
Related Product Information for anti-TG antibody
Thyroglobulin (TG) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
298,383 Da
NCBI Official Full Name
thyroglobulin
NCBI Official Synonym Full Names
thyroglobulin
NCBI Official Symbol
TG
NCBI Official Synonym Symbols
TGN; AITD3
NCBI Protein Information
thyroglobulin
UniProt Protein Name
Thyroglobulin
Protein Family
UniProt Gene Name
TG
UniProt Synonym Gene Names
Tg
UniProt Entry Name
THYG_HUMAN
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Product Notes
The TG tg (Catalog #AAA245003) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 2H11] (IgG1) to Human TG / Thyroglobulin reacts with Dog, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TG / Thyroglobulin can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA). ELISA, IHC-Fr (1:50 - 1:200), IHC-P (5 ug/ml), IP, WB (1:400) Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib.. Researchers should empirically determine the suitability of the TG tg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TG / Thyroglobulin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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