Rabbit anti-Human VAMP2 Polyclonal Antibody | anti-VAMP2 antibody

Anti-VAMP2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101771

• Gene Names: VAMP2; SYB2; VAMP-2
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: VAMP2; Polyclonal Antibody; Anti-VAMP2 Antibody; Rabbit Polyclonal; VAMP2 Antibody; Rabbit PAb; Antigen Affinity Purified; vesicle-associated membrane protein 2 (synaptobrevin 2); Anti-SYB2 Antibody; Anti-VAMP-2 Antibody; anti-VAMP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human VAMP2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-VAMP2 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:500-1:2000; ICC/IF: 1:300-1:10,000
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human VAMP2
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human VAMP2, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-VAMP2 rabbit polyclonal antibody at 1:500 dilutionLane A: Ratbrain Whole Cell LysateLane B: Mousebrain Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:13 kDaObserved band size:17 kDa(We are unsure as to the identity of these extra bands.))

Immunohistochemistry (IHC)

(Immunochemical staining of human VAMP2 in human brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human VAMP2 in human adrenal gland with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunofluorescence (IF)

(Immunofluorescence staining of VAMP2 in U87MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human VAMP2 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)

Related Product Information for anti-VAMP2 antibody

Vesicle-associated membrane protein 2 (VAMP2), a member of soluble N-ethylmaleimide sensitive factor attachment protein receptor (SNAREs) is required for membrane fusion. The transmembrane domains (TMDs) of VAMP2 plays a critical role in membrane fusion and that the structural mobility provided by the central small amino acids is crucial for exocytosis by influencing the molecular re-arrangements of the lipid membrane that are necessary for fusion pore opening and expansion. Moreover, VAMP2 is the main vesicle associated membrane protein (VAMP) isoform functionally involved in antibody secretion.

NCBI and Uniprot Product Information

• NCBI GI #: 288558837
• NCBI GeneID: 6844
• UniProt Accession #: P63027; P19065; Q9BUC2
• Molecular Weight: 13kDa
• NCBI Official Full Name: Vesicle-associated membrane protein 2
• NCBI Official Synonym Full Names: vesicle-associated membrane protein 2 (synaptobrevin 2)
• NCBI Official Symbol: VAMP2
• NCBI Official Synonym Symbols: SYB2; VAMP-2
• NCBI Protein Information: vesicle-associated membrane protein 2; synaptobrevin 2; synaptobrevin-2; vesicle associated membrane protein 2
• UniProt Protein Name: Vesicle-associated membrane protein 2
• Protein Family: Vesicle-associated membrane protein
• UniProt Gene Name: VAMP2
• UniProt Synonym Gene Names: SYB2; VAMP-2
• UniProt Entry Name: VAMP2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. It is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG. [provided by RefSeq, Jul 2008]

Uniprot Description

VAMP2: a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. Thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin.

Protein type: Motility/polarity/chemotaxis; Vesicle; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: zymogen granule membrane; neuron projection; synaptic vesicle membrane; intracellular membrane-bound organelle; clathrin-coated vesicle; integral to plasma membrane; secretory granule membrane; trans-Golgi network; cytosol; secretory granule; SNARE complex; synaptic vesicle; membrane; perinuclear region of cytoplasm; plasma membrane; synapse; cytoplasmic vesicle; cell junction

Molecular Function: calmodulin binding; SNAP receptor activity; protein binding; SNARE binding; protein self-association; syntaxin binding; syntaxin-1 binding; phospholipid binding; calcium-dependent protein binding

Biological Process: exocytosis; natural killer cell degranulation; neurotransmitter secretion; neutrophil degranulation; pathogenesis; calcium ion-dependent exocytosis; vesicle fusion; regulation of exocytosis; vesicle-mediated transport; synaptic transmission; protein transport; synaptic vesicle exocytosis; cellular protein metabolic process; glutamate secretion; cellular response to insulin stimulus; Golgi to plasma membrane protein transport; response to glucose stimulus; energy reserve metabolic process; eosinophil degranulation; protein complex assembly; post-Golgi vesicle-mediated transport; regulation of insulin secretion