Rabbit anti-Human, Mouse Vaccinia Related Kinase 1 Polyclonal Antibody | anti-VRK1 antibody

Vaccinia Related Kinase 1 (VRK1, Serine/threonine-protein kinase VRK1) (Biotin)

Cat. Num. AAA6505411

• Gene Names: VRK1; PCH1; PCH1A
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Vaccinia Related Kinase 1; Polyclonal Antibody; Vaccinia Related Kinase 1 (VRK1; Serine/threonine-protein kinase VRK1) (Biotin); anti-VRK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human VRK1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-VRK1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human VRK1 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-VRK1 Pab is used in Western blot to detect VRK1 in mouse lung tissue lysate (Lane 1) and HL-60 cell lysate (Lane 2).)

Western Blot (WB)

(Western blot analysis of VRK1 (arrow) using rabbit polyclonal VRK1 Antibody (E381). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the VRK1 gene.)

Product Categories/Family for anti-VRK1 antibody

Antibodies; Protein Kinases

References

1. Nezu, J., et al., Genomics 45(2):327-331 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 109730507
• NCBI GeneID: 7443
• UniProt Accession #: Q99986; Q3SYL2
• Molecular Weight: 45,476 Da
• NCBI Official Full Name: Vaccinia related kinase 1
• NCBI Official Synonym Full Names: VRK serine/threonine kinase 1
• NCBI Official Symbol: VRK1
• NCBI Official Synonym Symbols: PCH1; PCH1A
• NCBI Protein Information: serine/threonine-protein kinase VRK1
• UniProt Protein Name: Serine/threonine-protein kinase VRK1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: VRK1

NCBI Description

This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN. [provided by RefSeq, Jul 2008]

Uniprot Description

Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Phosphorylates ATF2 which activates its transcriptional activity.