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Immunoprecipitation (IP) (UTF1 was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate 2 uL anti-UTF1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-UTF1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 37 kDa Observed band size :37 kDa)

Rabbit anti-Human UTF1 Polyclonal Antibody | anti-UTF1 antibody

Anti-UTF1 Antibody, Rabbit Polyclonal

Reactivity
Human
Applications
Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
UTF1; Polyclonal Antibody; Anti-UTF1 Antibody; Rabbit Polyclonal; UTF1 Antibody; Rabbit PAb; Antigen Affinity Purified; anti-UTF1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human UTF1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-UTF1 antibody
Immunoprecipitation (IP)
Application Notes
IP: 1-5uL/mg of lysate
Immunogen
A synthetic peptide corresponding to the center region of the Human UTF1
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human UTF1, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(UTF1 was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate 2 uL anti-UTF1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-UTF1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 37 kDa Observed band size :37 kDa)

Immunoprecipitation (IP) (UTF1 was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate 2 uL anti-UTF1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-UTF1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 37 kDa Observed band size :37 kDa)
Related Product Information for anti-UTF1 antibody
Undifferentiated embryonic cell transcription factor 1 (Utf1) is expressed in pluripotent embryonic stem cells (ESCs) and primordial germ cells (PGCs). Utf1 expression is directly controlled by pluripotency factors Oct4 and Sox2, which form a ternary complex with the Utf1 enhancer. UTF1 is a putative marker for undifferentiated spermatogonia in humans, rats and horses. Moreover, UTF1 is expressed in undifferentiated spermatogonia, and its antibody can be used as a putative marker for Spermatogonial stem cells (SSCs) in stallions.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36,439 Da
NCBI Official Full Name
undifferentiated embryonic cell transcription factor 1
NCBI Official Synonym Full Names
undifferentiated embryonic cell transcription factor 1
NCBI Official Symbol
UTF1
NCBI Protein Information
undifferentiated embryonic cell transcription factor 1
UniProt Protein Name
Undifferentiated embryonic cell transcription factor 1
UniProt Gene Name
UTF1

NCBI Description

The protein encoded by this gene is a leucine zipper-containing transcriptional coactivator that may link the upstream activator ATF2 with the basal transcription complex. The encoded protein is closely associated with chromatin and is required for the proper differentiation of embryonic carcinoma and embryonic stem cells. Found nearly exclusively in pluripotent cells, this protein can also serve as a transcriptional repressor. [provided by RefSeq, Nov 2015]

Uniprot Description

UTF1: Acts as a transcriptional coactivator of ATF2. Binds to the N-terminal region of ATF2. Associates with the TFIID complex through interaction with TBP.

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: nucleus

Molecular Function: protein binding; transcription coactivator activity

Biological Process: male gonad development; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter

Research Articles on UTF1

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Product Notes

The UTF1 utf1 (Catalog #AAA8102882) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-UTF1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's UTF1 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP). IP: 1-5uL/mg of lysate. Researchers should empirically determine the suitability of the UTF1 utf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UTF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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