Sheep INS (Insulin) ELISA Kit | INS elisa kit

Sheep INS (Insulin) ELISA Kit

Cat. Num. AAA2540366

• Reactivity: Sheep
• Synonyms: INS (Insulin); Sheep INS (Insulin) ELISA Kit; INS elisa kit

• Reactivity: Sheep
• Specificity: This kit recognizes Sheep INS in samples. No significant cross-reactivity or interference between Sheep INS and analogues was observed.
• Sequence Length: 110

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 3.125-200ng/mL
• Sensitivity: 1.875ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Sheep INS were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Sheep INS were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for INS elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Sheep INS concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Sheep INS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Sheep INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Sheep INS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Sheep INS. You can calculate the concentration of Sheep INS in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 386828
• Molecular Weight: 21,537 Da
• NCBI Official Full Name: insulin
• UniProt Protein Name: Insulin
• Protein Family: Insulin
• UniProt Gene Name: INS
• UniProt Entry Name: INS_HUMAN

Uniprot Description

Insulin: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Heterodimer of a B chain and an A chain linked by two disulfide bonds. Belongs to the insulin family.

Protein type: Secreted, signal peptide; Secreted; Hormone

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: endoplasmic reticulum lumen; ER-Golgi intermediate compartment membrane; extracellular region; extracellular space; Golgi lumen; Golgi membrane; transport vesicle

Molecular Function: hormone activity; identical protein binding; insulin receptor binding; insulin-like growth factor receptor binding; protease binding; protein binding

Biological Process: activation of NF-kappaB transcription factor; activation of protein kinase B; acute-phase response; alpha-beta T cell activation; cell-cell signaling; cellular protein metabolic process; ER to Golgi vesicle-mediated transport; fatty acid homeostasis; G-protein coupled receptor protein signaling pathway; glucose homeostasis; glucose transport; insulin receptor signaling pathway; MAPKKK cascade; negative regulation of acute inflammatory response; negative regulation of fatty acid metabolic process; negative regulation of glycogen catabolic process; negative regulation of lipid catabolic process; negative regulation of NAD(P)H oxidase activity; negative regulation of protein catabolic process; negative regulation of protein oligomerization; negative regulation of protein secretion; negative regulation of proteolysis; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cellular protein metabolic process; positive regulation of cytokine secretion; positive regulation of DNA replication; positive regulation of glucose import; positive regulation of glycogen biosynthetic process; positive regulation of glycolysis; positive regulation of insulin receptor signaling pathway; positive regulation of MAPKKK cascade; positive regulation of mitosis; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid autophosphorylation; positive regulation of protein kinase B signaling cascade; regulation of amino acid metabolic process; regulation of protein localization; regulation of protein secretion; regulation of transmembrane transporter activity; wound healing

Disease: Diabetes Mellitus, Insulin-dependent, 2; Diabetes Mellitus, Permanent Neonatal; Hyperproinsulinemia; Maturity-onset Diabetes Of The Young, Type 10