Rabbit anti-Mouse USP40 Polyclonal Antibody | anti-USP40 antibody

USP40, NT (USP40, Ubiquitin carboxyl-terminal hydrolase 40, Deubiquitinating enzyme 40, Ubiquitin thioesterase 40, Ubiquitin-specific-processing protease 40) (FITC)

Cat. Num. AAA6361952

• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: USP40; Polyclonal Antibody; NT (USP40; Ubiquitin carboxyl-terminal hydrolase 40; Deubiquitinating enzyme 40; Ubiquitin thioesterase 40; Ubiquitin-specific-processing protease 40) (FITC); Ubiquitin-specific-processing protease 40; anti-USP40 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-USP40 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: USP40 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74-103 amino acids from the N-terminal region of human USP40.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(USP40 Antibody (N-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the USP40 antibody detected the USP40 protein (arrow).)

Related Product Information for anti-USP40 antibody

Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP40 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes (Quesada et al., 2004 [PubMed 14715245]).

Product Categories/Family for anti-USP40 antibody

Antibodies; Ubiquitin

References

Wu, Y.R., et al. Clin. Chim. Acta 411 (13-14), 955-958 (2010): Li, Y., et al. Hum. Mutat. 27(10):1017-1023(2006). Hillier, L.W., et al. Nature 434(7034):724-731(2005). Quesada, V., et al. Biochem. Biophys. Res. Commun. 314(1):54-62(2004). Puente, X.S., et al. Nat. Rev. Genet. 4(7):544-558(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 41055953
• NCBI GeneID: 55230
• NCBI Accession #: NP_060688.1
• NCBI GenBank Nucleotide #: NM_018218.2
• UniProt Accession #: Q9NVE5; Q6NX38; Q70EL0
• Molecular Weight: 140,130 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 40
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 40
• NCBI Official Symbol: USP40
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 40; ubiquitin thioesterase 40; deubiquitinating enzyme 40; ubiquitin thiolesterase 40; ubiquitin specific protease 40; ubiquitin-specific-processing protease 40
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 40
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: USP40
• UniProt Entry Name: UBP40_HUMAN

NCBI Description

Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP40 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes (Quesada et al., 2004 [PubMed 14715245]).[supplied by OMIM, Mar 2008]

Uniprot Description

USP40: May be catalytically inactive. Belongs to the peptidase C19 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Cell adhesion; EC 3.4.19.12; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: cytoplasm; nucleus

Molecular Function: cysteine-type endopeptidase activity; ubiquitin-specific protease activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination