Rabbit anti-Human USP4 Polyclonal Antibody | anti-USP4 antibody

USP4 (Ubiquitin Specific Peptidase 4, EC 3.1.2.15, UNP, UNPH)

Cat. Num. AAA625178

• Gene Names: USP4; UNP; Unph; MGC149848; MGC149849
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: USP4; Polyclonal Antibody; USP4 (Ubiquitin Specific Peptidase 4; EC 3.1.2.15; UNP; UNPH); Anti -USP4 (Ubiquitin Specific Peptidase 4; anti-USP4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes Human Ubiquitin Specific Protease 4 (USP4). Species Crossreactivity: Human.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.1% sodium azide.

• Applicable Applications for anti-USP4 antibody: Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunohistochemistry.; Dilution: Immunohistochemistry: 5ug/ml
• Immunogen: Synthetic peptide, KLH-conjugated. Epitope: internal domain of human
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(USP4 (5ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human thymus, followed by biotinylated goat rabbit IgG secondary, alkaline phosphatase-streptavidin and chromogen.)

Product Categories/Family for anti-USP4 antibody

Antibodies; Abs to Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 40795665
• NCBI GeneID: 7375
• NCBI Accession #: NP_003354.2
• NCBI GenBank Nucleotide #: NM_003363.3
• UniProt Accession #: Q08AK7; Q13107; Q53FJ3; Q53H56
• Molecular Weight: 108,523 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 4 isoform a
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 4 (proto-oncogene)
• NCBI Official Symbol: USP4
• NCBI Official Synonym Symbols: UNP; Unph; MGC149848; MGC149849
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 4; OTTHUMP00000210715; OTTHUMP00000210716; OTTHUMP00000210717; ubiquitin thioesterase 4; deubiquitinating enzyme 4; ubiquitin thiolesterase 4; ubiquitous nuclear protein homolog; ubiquitin carboxyl-terminal esterase 4; ubiquitin-specific processing protease 4; ubiquitin-specific-processing protease 4; ubiquitin specific protease 4 (proto-oncogene)
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: USP4
• UniProt Entry Name: Q08AK7_HUMAN

NCBI Description

The protein encoded by this gene is a protease that deubiquitinates target proteins such as ADORA2A and TRIM21. The encoded protein shuttles between the nucleus and cytoplasm and is involved in maintaining operational fidelity in the endoplasmic reticulum. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

USP4: Hydrolase that deubiquitinates target proteins such as the receptor ADORA2A, PDPK1 and TRIM21. Deubiquitination of ADORA2A increases the amount of functional receptor at the cell surface. Plays a role in the regulation of quality control in the ER. Interacts with RB1 (both dephosphorylated and hypophosphorylated forms). Interacts with ADORA2A (via cytoplasmic C-terminus); the interaction is direct. Interacts with RB1, RBL1 and RBL2. Overexpressed in small cell tumors and adenocarcinomas of the lung compared to wild-type lung. Expressed in the hippocampal neurons. Belongs to the peptidase C19 family. USP4 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; EC 3.4.19.12; Ubiquitin conjugating system; Ubiquitin-specific protease; Protease

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: lysosome; cytoplasm; plasma membrane; nucleus

Molecular Function: identical protein binding; protein binding; metal ion binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; adenosine receptor binding

Biological Process: assembly of spliceosomal tri-snRNP; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; regulation of protein stability; negative regulation of protein ubiquitination