Typical Testing Data/Standard Curve (for reference only)
Monkey Interleukin 29 ELISA Kit | IL-29 elisa kit
Monkey Interleukin 29 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: IL-29 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IL-29 antibody and an IL-29-HRP conjugate. The assay sample and buffer are incubated together with IL-29-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-29 concentration since IL-29 from samples and IL-29-HRP conjugate compete for the anti-IL-29 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-29 from the sample, fewer sites are left to bind IL-29-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-29 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28A (IL28A), and interleukin 28B (IL28B) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA). [provided by RefSeq, Jul 2008]
Uniprot Description
IL29: Cytokine with immunomodulatory activity. May play a role in antiviral immunity. Up-regulates MHC class I antigen expression. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. Belongs to the IL-28/IL-29 family.
Protein type: Secreted; Cytokine; Cell cycle regulation; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 19q13.13
Cellular Component: extracellular space; extracellular region; interleukin-28 receptor complex
Molecular Function: cytokine activity; interleukin-28 receptor binding; receptor binding
Biological Process: positive regulation of immune response; positive regulation of transcription, DNA-dependent; positive regulation of JAK-STAT cascade; JAK-STAT cascade; negative regulation of T cell differentiation; negative regulation of cell proliferation; negative regulation of interleukin-13 production; positive regulation of interferon-gamma production; negative regulation of T-helper 2 type immune response; positive regulation of tyrosine phosphorylation of STAT protein; negative regulation of interleukin-5 production; positive regulation of MHC class I biosynthetic process; negative regulation of transcription, DNA-dependent; negative regulation of memory T cell differentiation; defense response to virus
Research Articles on IL-29
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Product Notes
The Monkey IL-29 ifnl1 (Catalog #AAA755121) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA755121 ELISA Kit recognizes Monkey IL-29. It is sometimes possible for the material contained within the vial of "Interleukin 29, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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