Western Blot (WB)
(Western blot analysis of USP30 expression in A549 (A); HeLa (B); THP1 (C) whole cell lysates.)
Rabbit USP30 Polyclonal Antibody | anti-USP30 antibody
USP30 Antibody
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Immunogen Affinity Chromatography
Synonyms
USP30; Polyclonal Antibody; USP30 Antibody; Purified Polyclonal USP30 Antibody; Polyclonal USP30 antibody; Anti-USP30 antibody; Ubiquitin carboxyl-terminal hydrolase 30 antibody; Deubiquitinating enzyme 30 antibody; Ubiquitin thioesterase 30 antibody; Ubiquitin-specific-processing protease 30 antibody; Ub-specific protease 30 antibody; anti-USP30 antibody
Host
Rabbit
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of USP30 protein
Purity/Purification
Immunogen Affinity Chromatography
Form/Format
Supplied in liquid form in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3 with 30% Glycerol and 0.01% Sodium Azide
Sequence Length
517
Applicable Applications for anti-USP30 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
WB: 1:500-1:1000
IF: 1:100-1:500
ICC: 1:100-1:500
IF: 1:100-1:500
ICC: 1:100-1:500
Immunogen
USP30 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human USP30 as the immunogen
Cross-Reactivity
Mouse, Rat, Dog, Monkey
Preparation and Storage
Store at 4 degree C for short term storage. For long term, aliquot and store at -20 degree C. Avoid repeat freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of USP30 expression in A549 (A); HeLa (B); THP1 (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of USP30 expression in A549 (A); HeLa (B); THP1 (C) whole cell lysates.)
Related Product Information for anti-USP30 antibody
USP30 is a deubiquitinating enzyme tethered to the mitochondrial outer membrane that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PARK2): hydrolyzes ubiquitin attached by parkin on target proteins, such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy.
Product Categories/Family for anti-USP30 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase 30 isoform 1
NCBI Official Synonym Full Names
ubiquitin specific peptidase 30
NCBI Official Symbol
USP30
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 30
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 30
Protein Family
UniProt Gene Name
USP30
UniProt Synonym Gene Names
Ub-specific protease 30
UniProt Entry Name
UBP30_HUMAN
NCBI Description
USP30, a member of the ubiquitin-specific protease family (see USP1, MIM 603478), is a novel mitochondrial deubiquitinating (DUB) enzyme (Nakamura and Hirose, 2008 [PubMed 18287522]).[supplied by OMIM, Dec 2008]
Uniprot Description
USP30: May participate in the maintenance of mitochondrial morphology. Belongs to the peptidase C19 family.
Protein type: Membrane protein, integral; EC 3.4.19.12; Protease; Ubiquitin-specific protease
Chromosomal Location of Human Ortholog: 12q24.11
Cellular Component: mitochondrial outer membrane; mitochondrion; integral to membrane
Molecular Function: cysteine-type endopeptidase activity; ubiquitin-specific protease activity
Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; mitochondrial fusion; mitochondrion degradation
Research Articles on USP30
Similar Products
Product Notes
The USP30 usp30 (Catalog #AAA5315757) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's USP30 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1:500-1:1000 IF: 1:100-1:500 ICC: 1:100-1:500. Researchers should empirically determine the suitability of the USP30 usp30 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP30, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
