Western Blot (WB)
(Western blot analysis of extracts of mouse eye tissue, using NR2E3 antibody.)
Rabbit NR2E3 Polyclonal Antibody | anti-NR2E3 antibody
NR2E3 Antibody
Gene Names
NR2E3; PNR; RNR; rd7; ESCS; RP37
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
NR2E3; Polyclonal Antibody; NR2E3 Antibody; ESCS; MGC49976; NR2 E3; Nr2e3; NR2E3_HUMAN; Nuclear receptor subfamily 2 group E member 3; Photoreceptor specific nuclear receptor; Photoreceptor-specific nuclear receptor; PNR; Rd 7; Rd7; Retina specific nuclear receptor; Retina-specific nuclear receptor; Retinal degeneration 7; RNR; RP37; anti-NR2E3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
NR2E3 antibody detects endogenous levels of NR2E3.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
410
Applicable Applications for anti-NR2E3 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human NR2E3.
Subcellular Location
Nucleus.
Tissue Specificity
Eye specific; found solely in the outer nuclear layer of the adult neurosensory retina, where the nuclei of cone and rod photoreceptors reside.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (92%), Bovine (100%), Horse (100%), Sheep (100%), Chicken (92%), Xenopus (83%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts of mouse eye tissue, using NR2E3 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of mouse eye tissue, using NR2E3 antibody.)
Related Product Information for anti-NR2E3 antibody
Function: Orphan nuclear receptor of retinal photoreceptor cells. Transcriptional factor that is an activator of rod development and repressor of cone development. Binds the promoter region of a number of rod-and cone-specific genes, including rhodopsin, M-and S-opsin and rod-specific phosphodiesterase beta subunit. Enhances rhodopsin expression. Represses M-and S-cone opsin expression.
Subunit Structure: Homodimer. Interacts with PIAS3; the interaction sumoylates NR2E3 and promotes repression of cone-specific gene transcription and activation of rod-specific genes (By similarity). Component of a complex that includes NR2E3, PIAS3, NRL, CRX and/or NR1D1. Binds NR1D1. Binds direcly in the complex with CRX, PIAS3 and NR1D1 (By similarity). Interacts (via the DNA-binding domain) with CRX (via its DNA binding domain); the interaction represses S-and M-cone opsin expression.
Post-translational Modifications: Di-and tri-sumoylated in developing retina. PIAS3-mediated sumoylation promotes repression of cone-specific gene expression and activation of rod-specific genes. Sumoylation on Lys-185 appears to be the main site (By similarity).
Similarity: Belongs to the nuclear hormone receptor family. NR2 subfamily.
Subunit Structure: Homodimer. Interacts with PIAS3; the interaction sumoylates NR2E3 and promotes repression of cone-specific gene transcription and activation of rod-specific genes (By similarity). Component of a complex that includes NR2E3, PIAS3, NRL, CRX and/or NR1D1. Binds NR1D1. Binds direcly in the complex with CRX, PIAS3 and NR1D1 (By similarity). Interacts (via the DNA-binding domain) with CRX (via its DNA binding domain); the interaction represses S-and M-cone opsin expression.
Post-translational Modifications: Di-and tri-sumoylated in developing retina. PIAS3-mediated sumoylation promotes repression of cone-specific gene expression and activation of rod-specific genes. Sumoylation on Lys-185 appears to be the main site (By similarity).
Similarity: Belongs to the nuclear hormone receptor family. NR2 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 43-45 kDa
Predicted: 45 kDa
Predicted: 45 kDa
NCBI Official Full Name
photoreceptor-specific nuclear receptor isoform b
NCBI Official Synonym Full Names
nuclear receptor subfamily 2 group E member 3
NCBI Official Symbol
NR2E3
NCBI Official Synonym Symbols
PNR; RNR; rd7; ESCS; RP37
NCBI Protein Information
photoreceptor-specific nuclear receptor
UniProt Protein Name
Photoreceptor-specific nuclear receptor
Protein Family
UniProt Gene Name
NR2E3
UniProt Synonym Gene Names
PNR; RNR
NCBI Description
This protein is part of a large family of nuclear receptor transcription factors involved in signaling pathways. Nuclear receptors have been shown to regulate pathways involved in embryonic development, as well as in maintenance of proper cell function in adults. Members of this family are characterized by discrete domains that function in DNA and ligand binding. This gene encodes a retinal nuclear receptor that is a ligand-dependent transcription factor. Defects in this gene are a cause of enhanced S cone syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
Orphan nuclear receptor of retinal photoreceptor cells. Transcriptional factor that is an activator of rod development and repressor of cone development. Binds the promoter region of a number of rod- and cone-specific genes, including rhodopsin, M- and S-opsin and rod-specific phosphodiesterase beta subunit. Enhances rhodopsin expression. Represses M- and S-cone opsin expression.
Research Articles on NR2E3
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Product Notes
The NR2E3 nr2e3 (Catalog #AAA9603659) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NR2E3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NR2E3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the NR2E3 nr2e3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NR2E3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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