Goat USP28 Polyclonal Antibody | anti-USP28 antibody

USP28 (Ubiquitin Carboxyl-terminal Hydrolase 28, Deubiquitinating Enzyme 28, Ubiquitin Thiolesterase 28, Ubiquitin-specific-processing Protease 28, KIAA1515)

Cat. Num. AAA6005398

• Reactivity: Bovine, Human
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: USP28; Polyclonal Antibody; USP28 (Ubiquitin Carboxyl-terminal Hydrolase 28; Deubiquitinating Enzyme 28; Ubiquitin Thiolesterase 28; Ubiquitin-specific-processing Protease 28; KIAA1515); Anti -USP28 (Ubiquitin Carboxyl-terminal Hydrolase 28; anti-USP28 antibody

• Host: Goat
• Reactivity: Bovine, Human
• Clonality: Polyclonal
• Specificity: Recognizes human USP28. Species sequence homology: Bovine.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

• Applicable Applications for anti-USP28 antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA and Immunohistochemistry.; Dilution: ELISA: 1:64,000; Immunohistochemistry (Paraffin): 2.5-3.8ug/ml
• Immunogen: Synthetic peptide corresponding to C-SVELKHYIQEDN, from USP28, at the internal region of the protein (NP_065937.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

((3.8ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

Related Product Information for anti-USP28 antibody

Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus.

Product Categories/Family for anti-USP28 antibody

Antibodies; Abs to Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 16507200
• NCBI GeneID: 57646
• NCBI Accession #: NP_065937.1
• NCBI GenBank Nucleotide #: NM_020886.2
• UniProt Accession #: Q96RU2; Q9P213; B0YJC0; B0YJC1
• Molecular Weight: 122,491 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 28
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 28
• NCBI Official Symbol: USP28
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 28; ubiquitin thioesterase 28; deubiquitinating enzyme 28; ubiquitin thiolesterase 28; ubiquitin specific protease 28; ubiquitin-specific-processing protease 28; ubiquitin carboxyl-terminal hydrolase 28 variant 1
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 28
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: USP28
• UniProt Synonym Gene Names: KIAA1515
• UniProt Entry Name: UBP28_HUMAN

NCBI Description

The ubiquitin-dependent protein degradation pathway is essential for proteolysis of intracellular proteins and peptides. Enzymes that remove ubiquitin from ubiquitin-conjugated peptides, like USP28, affect the fate and degradation of intracellular proteins and are essential for maintenance of cell-free ubiquitin pools (Valero et al., 2001).[supplied by OMIM, Mar 2008]

Uniprot Description

USP28: a peptidase of the C19 family. USP28 and FBXW7 regulate Myc protein stability in response to DNA damage. Two isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.19.12; Ubiquitin-specific protease; Protease

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: nucleoplasm; protein complex; nucleolus; nucleus

Molecular Function: protein binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity

Biological Process: cell proliferation; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; DNA damage checkpoint; response to ionizing radiation; DNA repair; response to DNA damage stimulus; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis