Testing Data
(Fig. 1. Apoptosis was induced in Jurkat cells with camptothecin (2 mM) for 6 hours in the presence of various concentrations of Caspase-Family Inhibitor, Z-VAD- FMK. Caspase activity was measured using Caspase-3 Fluorometric Assay Kit according to the kit instructions.)
Caspase Family Inhibitor, Z-VAD-FMK, Inhibitor
Caspase Family Inhibitor, Z-VAD-FMK
Purity
PurifiedTLC: one spot.
Synonyms
Caspase Family Inhibitor; Z-VAD-FMK; inhibitor
Purity/Purification
Purified
TLC: one spot.
TLC: one spot.
Form/Format
Supplied as a lyophilized powder. Reconstitute with 20mM DMSO.
Sequence
Z-Val-Ala-Asp(OME)-FMK
Application Notes
Suitable for use in inhibiting caspase activity in cell culture as well as in vitro studies.
Dilution: Inhibiting caspase activity in cell culture: 2-10uM
Dilution: Inhibiting caspase activity in cell culture: 2-10uM
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with DMSO. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(Fig. 1. Apoptosis was induced in Jurkat cells with camptothecin (2 mM) for 6 hours in the presence of various concentrations of Caspase-Family Inhibitor, Z-VAD- FMK. Caspase activity was measured using Caspase-3 Fluorometric Assay Kit according to the kit instructions.)
Testing Data
(Fig. 1. Apoptosis was induced in Jurkat cells with camptothecin (2 mM) for 6 hours in the presence of various concentrations of Caspase-Family Inhibitor, Z-VAD- FMK. Caspase activity was measured using Caspase-3 Fluorometric Assay Kit according to the kit instructions.)
Related Product Information for Caspase Family Inhibitor, Z-VAD-FMK, inhibitor
A synthetic peptide that irreversibly inhibits activity of caspase family proteases and blocks apoptosis. The inhibitor is designed as a methyl ester to facilitate cell permeability.
Product Categories/Family for Caspase Family Inhibitor, Z-VAD-FMK, inhibitor
Similar Products
Product Notes
The Caspase Family Inhibitor, Z-VAD-FMK (Catalog #AAA655359) is an Inhibitor and is intended for research purposes only. The product is available for immediate purchase. Suitable for use in inhibiting caspase activity in cell culture as well as in vitro studies. Dilution: Inhibiting caspase activity in cell culture: 2-10uM. Researchers should empirically determine the suitability of the Caspase Family Inhibitor, Z-VAD-FMK for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Z-Val-Ala- Asp(OME)-F MK. It is sometimes possible for the material contained within the vial of "Caspase Family Inhibitor, Z-VAD-FMK, Inhibitor" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
