Testing Data
(Figure: Detection of USP21 in HeLa nuclear fraction (10ug) by Western blotting using PAb to USP21 (human) at 1:1'000 dilution. Note: The bands at ~40kDa are likely to be a truncated forms of the USP21 protein as both these and the full length USP21 band are removed by peptide blockade using the antigenic peptide.)
Rabbit anti-Human USP21 Polyclonal Antibody | anti-USP21 antibody
USP21 (human) polyclonal antibody
Suggested dilutions/conditions may not be available for all applications.
Short term: 4 degree C
Stable for at least 1 year after receipt when stored at -20 degree C. Dilute with PBS, pH 7.2-7.4, containing 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used). Store diluted antibody at +4 degree C, do not freeze and use within 1 month.
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20 degree C.
Testing Data
(Figure: Detection of USP21 in HeLa nuclear fraction (10ug) by Western blotting using PAb to USP21 (human) at 1:1'000 dilution. Note: The bands at ~40kDa are likely to be a truncated forms of the USP21 protein as both these and the full length USP21 band are removed by peptide blockade using the antigenic peptide.)
Testing Data
(Figure: Detection of USP21 in HeLa nuclear fraction (10ug) by Western blotting using PAb to USP21 (human) at 1:1'000 dilution. Note: The bands at ~40kDa are likely to be a truncated forms of the USP21 protein as both these and the full length USP21 band are removed by peptide blockade using the antigenic peptide.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the C19 peptidase family, also known as family 2 of ubiquitin carboxy-terminal hydrolases. The encoded protein cleaves ubiquitin from ubiquitinated proteins for recycling in intracellular protein degradation. The encoded protein is also able to release NEDD8, a ubiquitin-like protein, from NEDD8-conjugated proteins. This gene has been referred to as USP16 and USP23 but is now known as USP21. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2016]
Uniprot Description
USP21: Deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A releaves the repression of di- and trimethylation of histone H3 at 'Lys-4', resulting in regulation of transcriptional initiation. Regulates gene expression via histone H2A deubiquitination. Also capable of removing NEDD8 from NEDD8 conjugates but has no effect on Sentrin-1 conjugates. Belongs to the peptidase C19 family. USP21 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin conjugating system; Protease; EC 3.4.19.12; Ubiquitin-specific protease
Chromosomal Location of Human Ortholog: 1q22
Cellular Component: cytoplasm; nucleoplasm; plasma membrane
Molecular Function: cysteine-type peptidase activity; metal ion binding; NEDD8-specific protease activity; protein binding; transcription coactivator activity; ubiquitin-specific protease activity
Biological Process: histone deubiquitination; neurite development; positive regulation of transcription, DNA-dependent; transcription, DNA-dependent; ubiquitin-dependent protein catabolic process
Research Articles on USP21
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Product Notes
The USP21 usp21 (Catalog #AAA565316) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The USP21 (human) polyclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's USP21 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot (1:1,000) Suggested dilutions/conditions may not be available for all applications. Researchers should empirically determine the suitability of the USP21 usp21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP21, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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