Rabbit USP21 Polyclonal Antibody | anti-USP21 antibody

USP21 Polyclonal Antibody

Cat. Num. AAA3013650

• Gene Names: USP21; USP16; USP23
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Peptide affinity purified
• Synonyms: USP21; Polyclonal Antibody; USP21 Polyclonal Antibody; USP23; PP1490; Deubiquitinating enzyme 21; deubiquitinating enzyme 23; EC 3.1.2.15; ESTM28; MGC3394; NEDD8-specific protease; Ubiquitin carboxyl terminal hydrolase 21; ubiquitin carboxyl terminal hydrolase 23; Ubiquitin carboxyl-terminal hydrolase 21; ubiquitin specific peptidase 21; Ubiquitin specific processing protease 21; ubiquitin specific protease 16; ubiquitin specific protease 21; ubiquitin specific protease 23; Ubiquitin thioesterase 21; Ubiquitin thiolesterase 21; ubiquitin thiolesterase 23; ubiquitin-specific protease 23; formerly; Ubiquitin-specific-processing protease 21; UBP21; USP16 antibodyv Usp21; W53272; anti-USP21 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: USP21 polyclonal antibody detects endogenous levels of USP21 protein.
• Purity/Purification: Peptide affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 565

• Applicable Applications for anti-USP21 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500; ICC: 1:500-1:2,000; IHC: 1:50-1:200; FC/FACS: 1:50-1:100
• Immunogen: Peptide
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of USP21 on mouse thymus tissue lysate using anti-USP21 antibody at 1/200 dilution.)

Immunocytochemistry (ICC)

(ICC staining USP21 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-USP21 antibody

The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP21 (ubiquitin specific peptidase 21), also known as USP16 or USP23, is a 565 amino acid protein that belongs to the C19 peptidase family of ubiquitin carboxy-terminal hydrolases. Capable of removing ubiquitin from ubiquitinated proteins, USP21 plays a role in signal transduction and can also remove NEDD8 from NEDD8-conjugated proteins, possibly functioning to influence NEDD8-mediated protein proteolysis. Multiple isoforms of USP21 exist due to alternative splicing events.

NCBI and Uniprot Product Information

• NCBI GI #: 10720334
• NCBI GeneID: 27005
• UniProt Accession #: Q9UK80; Q9QZL6; B2GUX4
• Molecular Weight: 63kDa
• NCBI Official Full Name: Ubiquitin carboxyl-terminal hydrolase 21
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 21
• NCBI Official Symbol: USP21
• NCBI Official Synonym Symbols: USP16; USP23
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 21
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 21
• UniProt Gene Name: USP21
• UniProt Synonym Gene Names: USP23
• UniProt Entry Name: UBP21_HUMAN

NCBI Description

This gene encodes a member of the C19 peptidase family, also known as family 2 of ubiquitin carboxy-terminal hydrolases. The encoded protein cleaves ubiquitin from ubiquitinated proteins for recycling in intracellular protein degradation. The encoded protein is also able to release NEDD8, a ubiquitin-like protein, from NEDD8-conjugated proteins. This gene has been referred to as USP16 and USP23 but is now known as USP21. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2016]

Uniprot Description

USP21: Deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A releaves the repression of di- and trimethylation of histone H3 at 'Lys-4', resulting in regulation of transcriptional initiation. Regulates gene expression via histone H2A deubiquitination. Also capable of removing NEDD8 from NEDD8 conjugates but has no effect on Sentrin-1 conjugates. Belongs to the peptidase C19 family. USP21 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Protease; EC 3.4.19.12; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: cytoplasm; nucleoplasm; plasma membrane

Molecular Function: cysteine-type peptidase activity; metal ion binding; NEDD8-specific protease activity; protein binding; transcription coactivator activity; ubiquitin-specific protease activity

Biological Process: histone deubiquitination; neurite development; positive regulation of transcription, DNA-dependent; transcription, DNA-dependent; ubiquitin-dependent protein catabolic process