Rabbit anti-Human USP16 Polyclonal Antibody | anti-USP16 antibody

USP16 Antibody (Center)

Cat. Num. AAA9202222

• Gene Names: USP16; UBPM; UBP-M
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: USP16; Polyclonal Antibody; USP16 Antibody (Center); Ubiquitin carboxyl-terminal hydrolase 16 {ECO:0000255|HAMAP-Rule:MF_03062}; 341912 {ECO:0000255|HAMAP-Rule:MF_03062}; Deubiquitinating enzyme 16 {ECO:0000255|HAMAP-Rule:MF_03062}; Ubiquitin thioesterase 16 {ECO:0000255|HAMAP-Rule:MF_03062}; Ubiquitin-processing protease UBP-M; Ubiquitin-specific-processing protease 16 {ECO:0000255|HAMAP-Rule:MF_03062}; USP16 {ECO:0000255|HAMAP-Rule:MF_03062}; anti-USP16 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This USP16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 386-416 amino acids from the Central region of human USP16.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 386-416
• Sequence Length: 408

• Applicable Applications for anti-USP16 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of anti-USP16 Pab in HL60 cell line lysate (35ug/lane). USP16(arrow) was detected using the purified Pab.)

Related Product Information for anti-USP16 antibody

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Product Categories/Family for anti-USP16 antibody

Cell Biology

References

Puente, X.S., et al., Nat. Rev. Genet. 4(7):544-558 (2003).
Cai, S.Y., et al., Proc. Natl. Acad. Sci. U.S.A. 96(6):2828-2833 (1999).
D'Andrea, A., et al., Crit. Rev. Biochem. Mol. Biol. 33(5):337-352 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 50312664
• NCBI GeneID: 10600
• NCBI Accession #: NP_001001992.1; NP_001027582.1; NP_006438.1
• NCBI GenBank Nucleotide #: NM_001001992.1
• UniProt Accession #: Q9Y5T5; Q53GP7; Q53HA0; Q5VKN8; Q8NEL3; Q9H3E6; A8MU43; B3KN13; B4DFV8; B4DY37; D3DSD9
• Molecular Weight: 93570
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 16 isoform b
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 16
• NCBI Official Symbol: USP16
• NCBI Official Synonym Symbols: UBPM; UBP-M
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 16
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 16
• UniProt Gene Name: USP16
• UniProt Entry Name: UBP16_HUMAN

NCBI Description

This gene encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

USP16: a ubiquitous ubiquitin-processing protease that may de-ubiquitinate one or more critical proteins that are involved in the condensation of mitotic chromosomes, possibly acting selectively on histones H2A and H2B, the major ubiquitinated proteins of chromatin. Is phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition.

Protein type: Ubiquitin conjugating system; Protease; EC 3.4.19.12; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 21q22.11

Cellular Component: cytoplasm; nucleus

Molecular Function: ubiquitin binding; histone binding; zinc ion binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; transcription coactivator activity

Biological Process: regulation of transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; mitosis; transcription, DNA-dependent; cell division; positive regulation of transcription, DNA-dependent; positive regulation of translational elongation; positive regulation of transcription from RNA polymerase II promoter; cell cycle; response to DNA damage stimulus; histone deubiquitination; protein homotetramerization