Rabbit anti-Human USP16 Polyclonal Antibody | anti-USP16 antibody

USP16, NT (USP16, Ubiquitin carboxyl-terminal hydrolase 16, Deubiquitinating enzyme 16, Ubiquitin thioesterase 16, Ubiquitin-processing protease UBP-M, Ubiquitin-specific-processing protease 16) (PE)

Cat. Num. AAA6361871

• Gene Names: USP16; UBP-M
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: USP16; Polyclonal Antibody; NT (USP16; Ubiquitin carboxyl-terminal hydrolase 16; Deubiquitinating enzyme 16; Ubiquitin thioesterase 16; Ubiquitin-processing protease UBP-M; Ubiquitin-specific-processing protease 16) (PE); Ubiquitin-specific-processing protease 16; anti-USP16 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-USP16 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to a portion of amino acids within aa132-160 from the N-terminal region of human USP16, conjugated to KLH. Species sequence homology: monkey
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(USP16 Antibody (N-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the USP16 antibody detected the USP16 protein (arrow).)

Related Product Information for anti-USP16 antibody

This gene encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Product Categories/Family for anti-USP16 antibody

Antibodies; Ubiquitin

References

Gelsi-Boyer, V., et al. BMC Cancer 8, 299 (2008): Joo, H.Y., et al. Nature 449(7165):1068-1072(2007). Pai, M.T., et al. J. Mol. Biol. 370(2):290-302(2007). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 50312664
• NCBI GeneID: 10600
• NCBI Accession #: NP_001001992.1; NP_001027582.1
• NCBI GenBank Nucleotide #: NM_001001992.1
• UniProt Accession #: Q9Y5T5; Q53GP7; Q53HA0; Q5VKN8; Q8NEL3; Q9H3E6; A8MU43; B3KN13; B4DFV8; B4DY37; D3DSD9
• Molecular Weight: 93,570 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 16 isoform b
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 16
• NCBI Official Symbol: USP16
• NCBI Official Synonym Symbols: UBP-M
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 16; OTTHUMP00000096468; OTTHUMP00000096469; OTTHUMP00000096470; ubiquitin thioesterase 16; deubiquitinating enzyme 16; ubiquitin thiolesterase 16; ubiquitin specific protease 16; ubiquitin-processing protease UBP-M; u
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 16
• UniProt Gene Name: USP16
• UniProt Entry Name: UBP16_HUMAN