Rabbit anti-Human UQCRB Polyclonal Antibody | anti-UQCRB antibody

UQCRB, ID (UQCRB, UQBP, Cytochrome b-c1 complex subunit 7, Complex III subunit 7, Complex III subunit VII, QP-C, Ubiquinol-cytochrome c reductase complex 14kD protein) (FITC)

Cat. Num. AAA6361688

• Gene Names: UQCRB; QPC; QCR7; QP-C; UQBC; UQBP; UQPC; UQCR6; MC3DN3
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: UQCRB; Polyclonal Antibody; ID (UQCRB; UQBP; Cytochrome b-c1 complex subunit 7; Complex III subunit 7; Complex III subunit VII; QP-C; Ubiquinol-cytochrome c reductase complex 14kD protein) (FITC); Ubiquinol-cytochrome c reductase complex 14kD protein; anti-UQCRB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 111

• Applicable Applications for anti-UQCRB antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: UQCRB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-47 amino acids from the Central region of human UQCRB.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(UQCRB Antibody (Center) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the UQCRB antibody detected the UQCRB protein (arrow).)

Immunohistochemistry (IHC)

(UQCRB Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UQCRB Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-UQCRB antibody

This gene encodes a protein which is part of the ubiquinol-cytochrome c oxidoreductase complex which contains ten nuclear-encoded and one mitochondrial-encoded subunits. The encoded protein binds ubiquinone and participates in the transfer of electrons when ubiquinone is bound. Mutations in this gene are associated with mitochondrial complex III deficiency. A pseudogene has been described on the X chromosome.

Product Categories/Family for anti-UQCRB antibody

Antibodies; Enzymes; Cytochrome

References

Wang, L., et al. Cancer Epidemiol. Biomarkers Prev. 17(12):3558-3566(2008). Haut, S., et al. Hum. Genet. 113(2):118-122(2003). Malaney, S., et al. Cytogenet. Cell Genet. 73(4):297-299(1996). Suzuki, H., et al. J. Biol. Chem. 265(14):8159-8163(1990) Hosokawa, Y., et al. Biochem. Int. 21(1):41-44(1990).

NCBI and Uniprot Product Information

• NCBI GI #: 5454152
• NCBI GeneID: 7381
• NCBI Accession #: NP_006285.1
• NCBI GenBank Nucleotide #: NP_006285.1
• UniProt Accession #: P14927
• NCBI Official Full Name: cytochrome b-c1 complex subunit 7 isoform 1
• NCBI Official Synonym Full Names: ubiquinol-cytochrome c reductase binding protein
• NCBI Official Symbol: UQCRB
• NCBI Official Synonym Symbols: QPC; QCR7; QP-C; UQBC; UQBP; UQPC; UQCR6; MC3DN3
• NCBI Protein Information: cytochrome b-c1 complex subunit 7
• UniProt Protein Name: Cytochrome b-c1 complex subunit 7
• Protein Family: Cytochrome b-c1 complex
• UniProt Gene Name: UQCRB
• UniProt Synonym Gene Names: UQBP
• UniProt Entry Name: QCR7_HUMAN

NCBI Description

This gene encodes a subunit of the ubiquinol-cytochrome c oxidoreductase complex, which consists of one mitochondrial-encoded and 10 nuclear-encoded subunits. The protein encoded by this gene binds ubiquinone and participates in the transfer of electrons when ubiquinone is bound. This protein plays an important role in hypoxia-induced angiogenesis through mitochondrial reactive oxygen species-mediated signaling. Mutations in this gene are associated with mitochondrial complex III deficiency. Alternatively spliced transcript variants have been found for this gene. Related pseudogenes have been identified on chromosomes 1, 5 and X. [provided by RefSeq, Dec 2011]

Uniprot Description

Function: This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This component is involved in redox-linked proton pumping.

Subunit structure: The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1).

Subcellular location: Mitochondrion inner membrane.

Involvement in disease: Mitochondrial complex III deficiency, nuclear 3 (MC3DN3) [MIM:615158]: A disorder of the mitochondrial respiratory chain resulting in a highly variable phenotype depending on which tissues are affected. Clinical features include mitochondrial encephalopathy, psychomotor retardation, ataxia, severe failure to thrive, liver dysfunction, renal tubulopathy, muscle weakness and exercise intolerance.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.8

Sequence similarities: Belongs to the UQCRB/QCR7 family.

Caution: Was originally thought to be the ubiquinone-binding protein (QP-C).