Rabbit Rad50 Polyclonal Antibody | anti-Rad50 antibody

Rad50 (A708) Polyclonal Antibody

Cat. Num. AAA3002327

• Gene Names: RAD50; NBSLD; RAD502; hRad50
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Rad50; Polyclonal Antibody; Rad50 (A708) Polyclonal Antibody; DNA repair protein RAD50; hRAD50; RAD50; anti-Rad50 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Rad50 (A708) polyclonal antibody detects endogenous levels of Rad50 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1312

• Applicable Applications for anti-Rad50 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 670-722 of Human Rad50.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Rad50 (A708) polyclonal antibody at 1:500 dilutionLane1:786-O whole cell lysate (40ug) Lane2:U-87MG whole cell lysate (40ug) Lane3:SGC-7901 whole cell lysate (40ug) Lane4:SK-OVCAR3 whole cell lysate (40ug) Lane5:The Testis tissue lysate of Mouse (40ug) Lane6:The Testis tissue lysate of Rat (40ug) Lane7:C6 whole cell lysate (40ug))

Related Product Information for anti-Rad50 antibody

Rad52 family members (Rad50, Rad51B/C/D, Rad52, Rad54, MRE11) mediate DNA double-strand break repair (DSBR) for DNA damage that otherwise could cause cell death, mutation or neoplastic transformation. Rad51 (RECA, BRCC5) interacts with BRCA1 and BRCA2 to influence subcellular localization and cellular response to DNA damage. BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis from deregulation of Rad51. Rad52 forms a heptameric ring that binds single-stranded DNA ends and catalyzes DNA-DNA interaction necessary for the annealing of complementary strands.

NCBI and Uniprot Product Information

• NCBI GI #: 19924129
• NCBI GeneID: 10111
• NCBI Accession #: NP_005723.2
• NCBI GenBank Nucleotide #: NP_005723.2
• UniProt Accession #: Q92878
• Molecular Weight: ~ 153kDa
• NCBI Official Full Name: DNA repair protein RAD50
• NCBI Official Synonym Full Names: RAD50 double strand break repair protein
• NCBI Official Symbol: RAD50
• NCBI Official Synonym Symbols: NBSLD; RAD502; hRad50
• NCBI Protein Information: DNA repair protein RAD50
• UniProt Protein Name: DNA repair protein RAD50
• Protein Family: RAD50-interacting protein
• UniProt Gene Name: RAD50
• UniProt Synonym Gene Names: hRAD50
• UniProt Entry Name: RAD50_HUMAN

NCBI Description

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.[provided by RefSeq, Apr 2010]