Rabbit anti-Mouse UNC13B Polyclonal Antibody | anti-UNC13B antibody

UNC13B, NT (UNC13B, UNC13, Protein unc-13 homolog B, Munc13-2) (FITC)

Cat. Num. AAA6361446

• Gene Names: UNC13B; UNC13; MUNC13; Unc13h2
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: UNC13B; Polyclonal Antibody; NT (UNC13B; UNC13; Protein unc-13 homolog B; Munc13-2) (FITC); Munc13-2; anti-UNC13B antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-UNC13B antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: UNC13B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 262-292 amino acids from the N-terminal region of human UNC13B.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(UNC13B Antibody (N-term) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the UNC13B antibody detected the UNC13B protein (arrow).)

Related Product Information for anti-UNC13B antibody

This gene is expressed in the kidney cortical epithelial cells and is upregulated by hyperglycemia. The encoded protein shares a high level of similarity to the rat homolog, and contains 3 C2 domains and a diacylglycerol-binding C1 domain. Hyperglycemia increases the levels of diacylglycerol, which has been shown to induce apoptosis in cells transfected with this gene and thus contribute to the renal cell complications of hyperglycemia. Studies in other species also indicate a role for this protein in the priming step of synaptic vesicle exocytosis. [provided by RefSeq].

Product Categories/Family for anti-UNC13B antibody

Antibodies; Apoptosis

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Goldenberg, N.M., et al. Am. J. Physiol., Cell Physiol. 297 (4), C1053-C1058 (2009): Dimova, K., et al. Biochemistry 48(25):5908-5921(2009). Tregouet, D.A., et al. Diabetes 57(10):2843-2850(2008). Speight, P., et al. Traffic 6(10):858-865(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 110611226
• NCBI GeneID: 10497
• NCBI Accession #: NP_006368.3
• NCBI GenBank Nucleotide #: NM_006377.3
• UniProt Accession #: O14795; Q5VYM8
• Molecular Weight: 180,679 Da
• NCBI Official Full Name: protein unc-13 homolog B
• NCBI Official Synonym Full Names: unc-13 homolog B (C. elegans)
• NCBI Official Symbol: UNC13B
• NCBI Official Synonym Symbols: UNC13; MUNC13; Unc13h2
• NCBI Protein Information: protein unc-13 homolog B; munc13-2; homolog of rat Munc13 (diacylglycerol-binding)
• UniProt Protein Name: Protein unc-13 homolog B
• Protein Family: Protein
• UniProt Gene Name: UNC13B
• UniProt Synonym Gene Names: UNC13; munc13
• UniProt Entry Name: UN13B_HUMAN

Uniprot Description

UNC13B: Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity- depending refilling of readily releasable vesicle pool (RRP). Essential for synaptic vesicle maturation in a subset of excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. Belongs to the unc-13 family.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: Golgi apparatus; cytoplasm; presynaptic active zone; plasma membrane; terminal button; cell junction; cytosol; neuromuscular junction

Molecular Function: diacylglycerol binding; protein binding; signal transducer activity; metal ion binding; receptor activity

Biological Process: synaptic transmission, glutamatergic; synaptic transmission; glutamate secretion; apoptosis; neurotransmitter secretion; regulation of short-term neuronal synaptic plasticity; excretion; signal transduction; synaptic vesicle priming; neuromuscular junction development; synaptic vesicle docking during exocytosis