Rabbit anti-Human ULK2 Polyclonal Antibody | anti-ULK2 antibody

Rabbit Polyclonal (IgG) to Human ULK2

Cat. Num. AAA248976

• Gene Names: ULK2; ATG1B; Unc51.2
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: ULK2; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human ULK2; Anti-ULK2 Antibody (Internal) IHC-plus; ATG1B; KIAA0623; Unc-51-like kinase 2; Unc51.2; Human ULK2; anti-ULK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least two isoforms of ULK2 are known to exist; this antibody will detect both isoforms. ULK2 antibody is predicted to not cross-react with ULK1.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1036

• Applicable Applications for anti-ULK2 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (20 ug/ml), IHC-P (10 ug/ml), WB (1 ug/ml)
• Target Species: Human
• Immunogen Description: 17 amino acid peptide near the center of human ULK2.
• Immunogen: ULK2 antibody was raised against 17 amino acid peptide near the center of human ULK2.
• Antigen Modification: Internal
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-ULK2 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-ULK2 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunofluorescence (IF)

(Immunofluorescence of ULK2 in human brain tissue with ULK2 antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot analysis of ULK2 in human brain tissue lysate with ULK2 antibody at 1 ug/ml.)

Related Product Information for anti-ULK2 antibody

ULK2, a ULK kinase, has been suggested to be involved in a cell signaling pathway.

Product Categories/Family for anti-ULK2 antibody

Family: Protein Kinase; Subfamily: ULK

NCBI and Uniprot Product Information

• NCBI GI #: 217330557
• NCBI GeneID: 9706
• NCBI Accession #: NP_055498.3
• NCBI GenBank Nucleotide #: NM_014683.3
• UniProt Accession #: Q8IYT8; O75119; A8MY69
• Molecular Weight: 112,694 Da
• NCBI Official Full Name: serine/threonine-protein kinase ULK2
• NCBI Official Synonym Full Names: unc-51 like autophagy activating kinase 2
• NCBI Official Symbol: ULK2
• NCBI Official Synonym Symbols: ATG1B; Unc51.2
• NCBI Protein Information: serine/threonine-protein kinase ULK2
• UniProt Protein Name: Serine/threonine-protein kinase ULK2
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: ULK2
• UniProt Synonym Gene Names: KIAA0623
• UniProt Entry Name: ULK2_HUMAN

NCBI Description

This gene encodes a protein that is similar to a serine/threonine kinase in C. elegans which is involved in axonal elongation. The structure of this protein is similar to the C. elegans protein in that both proteins have an N-terminal kinase domain, a central proline/serine rich (PS) domain, and a C-terminal (C) domain. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Dec 2008]

Uniprot Description

ULK2: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and a negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK, also acts as a negative regulator of AMPK through phosphorylation of the AMPK subunits PRKAA1, PRKAB2 and PRKAG1. May phosphorylate ATG13/KIAA0652, FRS2, FRS3 and RPTOR; however such data need additional evidences. Not involved in ammonia-induced autophagy or in autophagic response of cerebellar granule neurons (CGN) to low potassium concentration. Plays a role early in neuronal differentiation and is required for granule cell axon formation: may govern axon formation via Ras-like GTPase signaling and through regulation of the Rab5-mediated endocytic pathways within developing axons. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.

Protein type: Autophagy; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Kinase, protein; Other group; ULK family

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: cytoplasmic vesicle membrane; pre-autophagosomal structure membrane; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: response to starvation; axon extension; protein amino acid autophosphorylation; regulation of autophagy; negative regulation of collateral sprouting; signal transduction; autophagic vacuole formation