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Testing Data (Gel: 10%SDS-PAGE Lysate: 40ug A431 cell Primary antibody: 1/800 dilution Secondary antibody dilution: 1/8000 Exposure time: 1 minute)

Rabbit anti-Human UGCG Polyclonal Antibody | anti-UGCG antibody

UGCG Antibody

Gene Names
UGCG; GCS; GLCT1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification.
Synonyms
UGCG; Polyclonal Antibody; UGCG Antibody; GCS; GLCT1; anti-UGCG antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total UGCG protein.
Purity/Purification
Antigen affinity purification.
Form/Format
Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
Concentration
1.9 mg/ml (varies by lot)
Sequence Length
394
Applicable Applications for anti-UGCG antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western blotting: 1:500-1:2000
Immunohistochemistry: 1:25-1:100
Immunogen Type
Recombinant Protein
Immunogen Description
Fusion protein corresponding to a region derived from internal residues of human UDP-glucose ceramide glucosyltransferase
Target Name
UGCG
Preparation and Storage
Store at -20 degree C

Testing Data

(Gel: 10%SDS-PAGE Lysate: 40ug A431 cell Primary antibody: 1/800 dilution Secondary antibody dilution: 1/8000 Exposure time: 1 minute)

Testing Data (Gel: 10%SDS-PAGE Lysate: 40ug A431 cell Primary antibody: 1/800 dilution Secondary antibody dilution: 1/8000 Exposure time: 1 minute)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using MBS9412231 at dilution 1/40.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using MBS9412231 at dilution 1/40.)
Related Product Information for anti-UGCG antibody
Glycosphingolipids (GSLs) are a group of membrane components that contain lipid and sugar moieties. They are present in essentially all animal cells and are believed to have important roles in various cellular processes. UDP-glucose ceramide glucosyltransferase catalyzes the first glycosylation step in glycosphingolipid biosynthesis. The product, glucosylceramide, is the core structure of more than 300 GSLs. UGCG is widely expressed and transcription is upregulated during keratinocyte differentiation.
Product Categories/Family for anti-UGCG antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
SDS-PAGE MW: 45kd
NCBI Official Full Name
ceramide glucosyltransferase
NCBI Official Synonym Full Names
UDP-glucose ceramide glucosyltransferase
NCBI Official Symbol
UGCG
NCBI Official Synonym Symbols
GCS; GLCT1
NCBI Protein Information
ceramide glucosyltransferase
UniProt Protein Name
Ceramide glucosyltransferase
UniProt Gene Name
UGCG
UniProt Synonym Gene Names
GCS
UniProt Entry Name
CEGT_HUMAN

NCBI Description

This gene encodes an enzyme that catalyzes the first glycosylation step in the biosynthesis of glycosphingolipids, which are membrane components containing lipid and sugar moieties. The product of this reaction is glucosylceramide, which is the core structure of many glycosphingolipids. [provided by RefSeq, Dec 2014]

Uniprot Description

UGCG: Catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. May also serve as a flippase. Belongs to the glycosyltransferase 2 family.

Protein type: EC 2.4.1.80; Lipid Metabolism - sphingolipid; Membrane protein, integral; Transferase; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 9q31

Cellular Component: Golgi membrane; membrane; integral to membrane

Molecular Function: ceramide glucosyltransferase activity

Biological Process: epidermis development; sphingolipid metabolic process; glycosphingolipid biosynthetic process; glycosphingolipid metabolic process; glucosylceramide biosynthetic process

Research Articles on UGCG

Similar Products

Product Notes

The UGCG ugcg (Catalog #AAA9412231) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UGCG Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's UGCG can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western blotting: 1:500-1:2000 Immunohistochemistry: 1:25-1:100. Researchers should empirically determine the suitability of the UGCG ugcg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UGCG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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