SDS-Page
Microtubule Associated Protein 4 Recombinant Protein | MAP4 recombinant protein
Recombinant Microtubule Associated Protein 4 (MAP4)
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
>95%
Synonyms
Microtubule Associated Protein 4; Recombinant Microtubule Associated Protein 4 (MAP4); MAP4 recombinant protein
Host
Host: E Coli
Source: Prokaryotic expression
Source: Prokaryotic expression
Purity/Purification
>95%
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Concentration
4.2 (varies by lot)
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- ADLSLVDAL TEPPPEIEGE IKRDFMAALE AEPYDDIVGE TVEKTEFIPL LDGDEKSGNS ESKRKPCVDT SQVEDIPSSK PTLLANGDHG VEGNNTTGSP TDFLVENVDY EDYQNSQSWP EDASFCFQPQ QVLDTNQADP FNVHHDDGLA DLLFVSSGPT NASAFIEENN PLEDSYGVLP CDSFAPTAVV SQEWSVGAPD SPHSEPCVSP EVTIETAQPA TELSKAVDLE SVKEQLPAKA LEMMAGQTTD
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- ADLSLVDAL TEPPPEIEGE IKRDFMAALE AEPYDDIVGE TVEKTEFIPL LDGDEKSGNS ESKRKPCVDT SQVEDIPSSK PTLLANGDHG VEGNNTTGSP TDFLVENVDY EDYQNSQSWP EDASFCFQPQ QVLDTNQADP FNVHHDDGLA DLLFVSSGPT NASAFIEENN PLEDSYGVLP CDSFAPTAVV SQEWSVGAPD SPHSEPCVSP EVTIETAQPA TELSKAVDLE SVKEQLPAKA LEMMAGQTTD
Sequence Length
1152
Applicable Applications for MAP4 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Predicted Molecular Mass
30.7kDa
Accurate Molecular Mass (KD)
50kDa
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Expression System
Prokaryotic expression
Tag
two N-terminal Tags, His-tag and T7-tag
Organism Species
Rattus norvegicus (Rat)
Fragment
Val22~Cys167
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
10,441 Da
NCBI Official Full Name
microtubule-associated protein 4
UniProt Protein Name
Microtubule-associated protein 4
Protein Family
UniProt Gene Name
MAP4
UniProt Synonym Gene Names
MAP-4
UniProt Entry Name
MAP4_HUMAN
Uniprot Description
MAP4: a microtuble-associated protein detected predominately in nonneuronal cells. May play a role in microtubule stabilization as well as promoting microtubule assembly. Four alternatively spliced variants have been described.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 3p21
Cellular Component: microtubule cytoskeleton; microtubule; microtubule associated complex; cytoplasm; plasma membrane
Molecular Function: protein binding; microtubule binding; structural molecule activity
Biological Process: mitotic spindle organization and biogenesis; cell division; microtubule sliding; establishment of spindle orientation
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Product Notes
The MAP4 map4 (Catalog #AAA2029590) is a Recombinant Protein produced from Host: E Coli Source: Prokaryotic expression and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Microtubule Associated Protein 4 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the MAP4 map4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.MGSSHHHHH H SSGLVPRGSH MASMTGGQQM GRGSEF- ADLSLVDAL TEPPPEIEGE IKRDFMAALE AEPYDDIVGE TVEKTEFIPL LDGDEKSGNS ESKRKPCVDT SQVEDIPSSK PTLLANGDHG VEGNNTTGSP TDFLVENVDY EDYQNSQSWP EDASFCFQPQ QVLDTNQADP FNVHHDDGLA DLLFVSSGPT NASAFIEENN PLEDSYGVLP CDSFAPTAVV SQEWSVGAPD SPHSEPCVSP EVTIETAQPA TELSKAVDLE SVKEQLPAKA LEMMAGQTTD
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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