Rabbit anti-Human, Mouse UFD1L Polyclonal Antibody | anti-UFD1L antibody

UFD1L Polyclonal Antibody

Cat. Num. AAA3011733

• Gene Names: UFD1; UFD1L
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: UFD1L; Polyclonal Antibody; UFD1L Polyclonal Antibody; Ubiquitin fusion degradation protein 1 homolog; UB fusion protein 1; anti-UFD1L antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: UFD1L polyclonal antibody detects endogenous levels of UFD1L protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 266

• Applicable Applications for anti-UFD1L antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant full length Human UFD1L.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of UFD1L polyclonal antibody)

Related Product Information for anti-UFD1L antibody

Ubiquitin-mediated proteolysis requires the transfer of ubiquitin (Ub) to lysine groups on selected cellular proteins, which then potentiates the proteolytic degradation of these protein conjugates by the 26S proteasome. Ub-fusions are cleaved by Ub-specific processing proteases (UBps) or alternatively by the Ub-fusion degradation (UFD) pathway. The UBP pathway targets the C-terminal glycine residue on Ub that is involved in the formation of Ub-conjugates, while UFD proteins preferentially cleave Ub-conjugated proteins that contain an amino acid substitution at this glycine residue. The UFD1 protein was originally characterized in the yeast S. cerevisiae and subsequently, the human homolog UFD1 or UFD1L was identified. In vitro, UFD1 attenuates the degradation of Ub-fusions, which have a proline or valine residue substituted at the Gly76 moiety, by the selective multiubiquitination of the Ub chain of the Ub-conjugate. Mutations within the UFD1 gene are implicated in the development of CATCH22 syndrome, which is characterized by cardiac defects, cleft palate and hypocalcemia, suggesting that this proteolytic pathway may be involved in the progression of these developmental defects.

NCBI and Uniprot Product Information

• NCBI GI #: 241982785
• NCBI GeneID: 7353
• NCBI Accession #: NP_001030324.2
• NCBI GenBank Nucleotide #: NP_001030324.2
• UniProt Accession #: Q92890
• Molecular Weight: ~ 35kDa
• NCBI Official Full Name: ubiquitin recognition factor in ER-associated degradation protein 1 isoform B
• NCBI Official Synonym Full Names: ubiquitin recognition factor in ER associated degradation 1
• NCBI Official Symbol: UFD1
• NCBI Official Synonym Symbols: UFD1L
• NCBI Protein Information: ubiquitin recognition factor in ER-associated degradation protein 1
• UniProt Protein Name: Ubiquitin fusion degradation protein 1 homolog
• UniProt Gene Name: UFD1L
• UniProt Synonym Gene Names: UB fusion protein 1
• UniProt Entry Name: UFD1_HUMAN

NCBI Description

The protein encoded by this gene forms a complex with two other proteins, nuclear protein localization-4 and valosin-containing protein, and this complex is necessary for the degradation of ubiquitinated proteins. In addition, this complex controls the disassembly of the mitotic spindle and the formation of a closed nuclear envelope after mitosis. Mutations in this gene have been associated with Catch 22 syndrome as well as cardiac and craniofacial defects. Alternative splicing results in multiple transcript variants encoding different isoforms. A related pseudogene has been identified on chromosome 18. [provided by RefSeq, Jun 2009]