UCP2 Polyclonal Antibody | anti-UCP2 antibody

Goat Polyclonal to Human UCP2

Cat. Num. AAA242664

• Gene Names: UCP2; UCPH; BMIQ4; SLC25A8
• Reactivity: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Xenopus.; Predicted Reactivity: Zebrafish
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: UCP2; Polyclonal Antibody; Goat Polyclonal to Human UCP2; Anti-UCP2 Antibody (Internal) IHC-plus; BMIQ4; UCP 2; Uncoupling protein 2; UCPH; SLC25A8; Human UCP2; anti-UCP2 antibody

• Host: UCP2 antibody was produced in Goat
• Reactivity: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Xenopus.; Predicted Reactivity: Zebrafish
• Clonality: Polyclonal
• Specificity: Human UCP2.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/mL (varies by lot)
• Sequence Length: 309

• Applicable Applications for anti-UCP2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: IHC- Paraffin(3.75 ug/ml); Western blot (1-3ug/mL); ELISA (1:32000); ; Optimal dilution to be determined by the researcher).
• Immunogen Species: UCP2 antibody was raised against Human.
• Antigen Type: Synthetic peptide
• Immunogen: UCP2 antibody was raised against synthetic peptide C-DSVKQFYTKGSEH from an internal region of human UCP2 (NP_003346.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Xenopus, Stickleback (100%); Smelt, Zebrafish (92%); Trout, Salmon (85%).
• Antigen Modification: Internal
• Epitope: aa101-113
• Usage summary: Immunohistochemistry: MBS242664 was validated for use in imunohistochemistry on a panel of 21 formalin-fixed, paraffin- embedded (FFPE)human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody,slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specifity. The optimal working concentration for MBS242664 was determined to be 3.75 ug/mL.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at -20°C. Avoid freezing and thawing.

Immunohistochemistry (IHC)

(Anti-UCP2 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.)

Immunohistochemistry (IHC)

(Anti-UCP2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.)

Immunohistochemistry (IHC)

(Anti-UCP2 antibody IHC of human adipocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.)

Western Blot (WB)

(Antibody (0.5 ug/ml) staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Product Categories/Family for anti-UCP2 antibody

Family: Transporter; Subfamily: Mitochondrial carrier

NCBI and Uniprot Product Information

• NCBI GI #: 13259541
• NCBI GeneID: 7351
• NCBI Accession #: NP_003346.2
• NCBI GenBank Nucleotide #: NM_003355.2
• UniProt Accession #: P55851; Q4PJH8; Q53HM3
• NCBI Official Full Name: mitochondrial uncoupling protein 2
• NCBI Official Synonym Full Names: uncoupling protein 2 (mitochondrial, proton carrier)
• NCBI Official Symbol: UCP2
• NCBI Official Synonym Symbols: UCPH; BMIQ4; SLC25A8
• NCBI Protein Information: mitochondrial uncoupling protein 2; solute carrier family 25 member 8
• UniProt Protein Name: Mitochondrial uncoupling protein 2
• UniProt Gene Name: UCP2
• UniProt Synonym Gene Names: SLC25A8; UCP 2
• UniProt Entry Name: UCP2_HUMAN

NCBI Description

Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed in many tissues, with the greatest expression in skeletal muscle. It is thought to play a role in nonshivering thermogenesis, obesity and diabetes. Chromosomal order is 5'-UCP3-UCP2-3'. [provided by RefSeq, Jul 2008]

Uniprot Description

UCP2: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Belongs to the mitochondrial carrier family.

Protein type: Transporter; Membrane protein, integral; Mitochondrial; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: mitochondrial inner membrane; integral to membrane

Biological Process: cellular metabolic process; proton transport; response to superoxide; cellular response to insulin stimulus; regulation of mitochondrial membrane potential; cellular response to amino acid starvation; transport; response to hypoxia; female pregnancy; mitochondrial transport; negative regulation of apoptosis; aging

Disease: Body Mass Index Quantitative Trait Locus 4