CFL2 / cofilin 2 / ADF Recombinant Protein | CFL2 recombinant protein

Recombinant Human CFL2 / cofilin 2 / ADF Protein (His tag)

Cat. Num. AAA2545775

• Gene Names: CFL2; NEM7
• Purity: > 98 % as determined by SDS-PAGE
• Synonyms: CFL2 / cofilin 2 / ADF; Recombinant Human CFL2 / cofilin 2 / ADF Protein (His tag); NEM7; CFL2 recombinant protein

• Host: E Coli
• Purity/Purification: > 98 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.5
• Sequence Length: 149

• Application Notes: The recombinant human CFL2 consisting of 180 amino acids and has a calculated molecular mass of 20.4 kDa. The apparent molecular mass of the protein is approximately 21 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CFL2 recombinant protein

Background: Cofilin 2 (muscle), also known as CFL2, is a member of cofilin family of the actin-binding protein superfamily. Cofilin2 shows significant homology to the other two members: cofilin 1 and DSTN, through its entire sequence, and contains residues conserved among the cofilin family that are responsible for actin-binding. Cofilin 2 (CFL2) is an important regulator of striated myocyte function. Purified cofilin 2 depolymerized actin filaments in a dose- and pH-dependent manner and reduced the apparent viscosity of an actin solution, although they did not co-sediment with actin filaments at all. Cofilin2 is not expressed in vegetative cells, but is transiently induced during the aggregation stage of development, whereas cofilin 1 was predominantly expressed in vegetative cells. 

Description: A DNA sequence encoding the human CFL2 (Q9Y281-1) (Ala 2-Leu 166) was expressed, with a polyhistidine tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 343887344
• NCBI GeneID: 1073
• NCBI Accession #: NP_001230574.1
• NCBI GenBank Nucleotide #: NM_001243645.1
• UniProt Accession #: Q9Y281; G3V5P4
• Molecular Weight: – Da
• NCBI Official Full Name: cofilin-2 isoform 2
• NCBI Official Synonym Full Names: cofilin 2
• NCBI Official Symbol: CFL2
• NCBI Official Synonym Symbols: NEM7
• NCBI Protein Information: cofilin-2
• UniProt Protein Name: Cofilin-2
• Protein Family: Cofilin
• UniProt Gene Name: CFL2

NCBI Description

This gene encodes an intracellular protein that is involved in the regulation of actin-filament dynamics. This protein is a major component of intranuclear and cytoplasmic actin rods. It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH-dependent manner. Mutations in this gene cause nemaline myopathy type 7, a form of congenital myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009]

Uniprot Description

Cofilin-2: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 14q13.1

Cellular Component: extracellular space; I band; Z disc

Molecular Function: actin filament binding; protein binding

Biological Process: actin filament depolymerization; positive regulation of actin filament depolymerization

Disease: Nemaline Myopathy 7