Western Blot (WB)
(Western Blot analysis of UCP2 expression in transfected 293T cell line by UCP2 polyclonal antibody. Lane 1: UCP2 transfected lysate (33.2kD). Lane 2: Non-transfected lysate.)
Rabbit anti-Human UCP2 Polyclonal Antibody | anti-UCP2 antibody
UCP2 (Mitochondrial Uncoupling Protein 2, UCP 2, Solute Carrier Family 25 Member 8, UCPH, SLC25A8) (AP)
Gene Names
UCP2; UCPH; BMIQ4; SLC25A8
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
UCP2; Polyclonal Antibody; UCP2 (Mitochondrial Uncoupling Protein 2; UCP 2; Solute Carrier Family 25 Member 8; UCPH; SLC25A8) (AP); anti-UCP2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human UCP2.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-UCP2 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length human UCP2, aa1-309 (NP_003346.2).
Immunogen Sequence
MVGFKATDVPPTATVKFLGAGTAACIADLITFPLDTAKVRLQIQGESQGPVRATASAQYRGVMGTILTMVRTEGPRSLYNGLVAGLQRQMSFASVRIGLYDSVKQFYTKGSEHASIGSRLLAGSTTGALAVAVAQPTDVVKVRFQAQARAGGGRRYQSTVNAYKTIAREEGFRGLWKGTSPNVARNAIVNCAELVTYDLIKDALLKANLMTDDLPCHFTSAFGAGFCTTVIASPVDVVKTRYMNSALGQYSSAGHCALTMLQKEGPRAFYKGFMPSFLRLGSWNVVMFVTYEQLKRALMAACTSREAPF
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of UCP2 expression in transfected 293T cell line by UCP2 polyclonal antibody. Lane 1: UCP2 transfected lysate (33.2kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of UCP2 expression in transfected 293T cell line by UCP2 polyclonal antibody. Lane 1: UCP2 transfected lysate (33.2kD). Lane 2: Non-transfected lysate.)
Related Product Information for anti-UCP2 antibody
White and brown adipose tissues (WAT and BAT, respectively) play a central role in body weight and energy expenditure. WAT is the major site for energy storage via triglyceride synthesis and mobilization via lipolysis. Uncoupling proteins (UCP1-5) are a family of mitochondria transprt proteins that play a critical role in thermoregulatory heat production and maintenance of basal metabolic rate. BAT is able to dissipate energy as heat via uncoupled mitochondrial respiration by a mitochondrial anion carrier, uncoupling protein 1 (UCP1). UCP1 is predicted to contain 6 transmembrane (TM) domains, a putative purine nucleotide-binding domain (PNBD) and three-mitochondrial energy transfer protein domains (ETPDs). Both amino and C-termini are predicted to be cytoplasmic. Mouse/rat UCP2 is a 309aa (human 309aa, chromosome 7; ~95% homology) mitochondrial uncoupling protein. It is only 59% homologous with UCP1 found in brown adipose tissues. UCP2 has wide tissue distribution in mouse tissues (brain, kidney, liver, brown adipose tissue, heart and muscle). UCP2 may play a critical role in energy balance, body weight and thermoregulation.
Product Categories/Family for anti-UCP2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,229 Da
NCBI Official Full Name
mitochondrial uncoupling protein 2
NCBI Official Synonym Full Names
uncoupling protein 2 (mitochondrial, proton carrier)
NCBI Official Symbol
UCP2
NCBI Official Synonym Symbols
UCPH; BMIQ4; SLC25A8
NCBI Protein Information
mitochondrial uncoupling protein 2; solute carrier family 25 member 8
UniProt Protein Name
Mitochondrial uncoupling protein 2
UniProt Gene Name
UCP2
UniProt Synonym Gene Names
SLC25A8; UCP 2
UniProt Entry Name
UCP2_HUMAN
Uniprot Description
UCP2: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Belongs to the mitochondrial carrier family.
Protein type: Membrane protein, multi-pass; Mitochondrial; Membrane protein, integral; Transporter
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: mitochondrial inner membrane; integral to membrane
Biological Process: cellular metabolic process; proton transport; response to superoxide; regulation of mitochondrial membrane potential; cellular response to insulin stimulus; transport; cellular response to amino acid starvation; response to hypoxia; female pregnancy; negative regulation of apoptosis; aging; mitochondrial transport
Disease: Body Mass Index Quantitative Trait Locus 4
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Product Notes
The UCP2 ucp2 (Catalog #AAA6397952) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UCP2 (Mitochondrial Uncoupling Protein 2, UCP 2, Solute Carrier Family 25 Member 8, UCPH, SLC25A8) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's UCP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the UCP2 ucp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UCP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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