Rabbit UCP2 Polyclonal Antibody | anti-UCP2 antibody

Anti-UCP2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101769

• Gene Names: UCP2; UCPH; BMIQ4; SLC25A8
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Applications: Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: UCP2; Polyclonal Antibody; Anti-UCP2 Antibody; Rabbit Polyclonal; UCP2 Antibody; Rabbit PAb; Antigen Affinity Purified; uncoupling protein 2 (mitochondrial; proton carrier); Anti-BMIQ4 Antibody; Anti-SLC25A8 Antibody; Anti-UCPH Antibody; anti-UCP2 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human UCP2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-UCP2 antibody: Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: ICC/IF: 1:100-1:500
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human UCP2
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human UCP2, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence staining of UCP2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human UCP2 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

Related Product Information for anti-UCP2 antibody

Uncoupling protein 2 (UCP2) is an ion/anion transporter located in the mitochondrial inner membrane, and has a crucial role in regulating oxidative stress, cellular metabolism, cell proliferation, and cell death. UCP2 uncouples the oxidative phosphorylation from ATP production by dissipating the proton gradient generated across the mitochondrial inner membrane. UCP2 is a predominant isoform of UCP that is expressed in the heart and other tissues of human and rodent tissues. Polymorphisms of the UCP2 gene have been associated with diabetes and obesity because UCP2 is involved in energy expenditure and insulin secretion. In rodents, UCP2 is involved in the control of alpha-and beta-cell mass as well as insulin and glucagon secretion. Moreover, UCP2 gene expression is often amplified in cancers, and increased UCP2 expression contributes to cancer growth, cancer metabolism, anti-apoptosis, and drug resistance.

References

Wu H, et al. (2019) Ucp2 protect the heart from myocardial ischemia/reperfusion injury via induction of mitochondrial autophagy. J Cell Biochem 120 (9): 15455-15466.Toda C, et al. (2014) Mitochondrial ucp2 in the central regulation of metabolism. Best Pract Res Clin Endocrinol Metab 28 (5): 757-764.Li J, et al. (2019) Ucp2 gene polymorphisms in obesity and diabetes, and the role of ucp2 in cancer. FEBS Lett 593 (18): 2525-2534.Broche B, et al. (2018) Mitochondrial protein ucp2 controls pancreas development. Diabetes 67 (1): 78-84.

NCBI and Uniprot Product Information

• NCBI GI #: 2497981
• NCBI GeneID: 7351
• UniProt Accession #: P55851; Q4PJH8; Q53HM3
• Molecular Weight: 309
• NCBI Official Full Name: Mitochondrial uncoupling protein 2
• NCBI Official Synonym Full Names: uncoupling protein 2 (mitochondrial, proton carrier)
• NCBI Official Symbol: UCP2
• NCBI Official Synonym Symbols: UCPH; BMIQ4; SLC25A8
• NCBI Protein Information: mitochondrial uncoupling protein 2; solute carrier family 25 member 8
• UniProt Protein Name: Mitochondrial uncoupling protein 2
• UniProt Gene Name: UCP2
• UniProt Synonym Gene Names: SLC25A8; UCP 2
• UniProt Entry Name: UCP2_HUMAN

NCBI Description

Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed in many tissues, with the greatest expression in skeletal muscle. It is thought to play a role in nonshivering thermogenesis, obesity and diabetes. Chromosomal order is 5'-UCP3-UCP2-3'. [provided by RefSeq, Jul 2008]

Uniprot Description

UCP2: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Belongs to the mitochondrial carrier family.

Protein type: Membrane protein, multi-pass; Mitochondrial; Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: mitochondrial inner membrane; integral to membrane

Biological Process: cellular metabolic process; proton transport; response to superoxide; regulation of mitochondrial membrane potential; cellular response to insulin stimulus; transport; cellular response to amino acid starvation; response to hypoxia; female pregnancy; negative regulation of apoptosis; aging; mitochondrial transport

Disease: Body Mass Index Quantitative Trait Locus 4