CD322 recombinant protein

CD322 Recombinant Protein

Cat. Num. AAA3004389

• Gene Names: JAM2; JAMB; CD322; JAM-B; VEJAM; PRO245; VE-JAM; C21orf43
• Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is >85% (by SDS-PAGE).
• Synonyms: CD322; CD322 Recombinant Protein; CD322 recombinant protein

• Host: E Coli
• Purity/Purification: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is >85% (by SDS-PAGE).
• Form/Format: PBS, 4M Urea, PH7.4
• Concentration: >=0.5mg/ml (varies by lot)

• Tag: His-tag
• Expression Vector: pet-22b(+)
• Restriction Site: NdeI-XhoI
• Preparation and Storage: Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.

Testing Data

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Related Product Information for CD322 recombinant protein

Background: Junctional adhesion protein that mediates heterotypic cell-cell interactions with its cognate receptor JAM3 to regulate different cellular processes. Plays a role in homing and mobilization of hematopoietic stem and progenitor cells within the bone marrow. At the surface of bone marrow stromal cells, it contributes to the retention of the hematopoietic stem and progenitor cells expressing JAM3. Plays a central role in leukocytes extravasation by facilitating not only transmigration but also tethering and rolling of leukocytes along the endothelium. Tethering and rolling of leukocytes are dependent on the binding by JAM2 of the integrin alpha-4/beta-1. Plays a role in spermatogenesis where JAM2 and JAM3, which are respectively expressed by Sertoli and germ cells, mediate an interaction between both cell types and play an essential role in the anchorage of germ cells onto Sertoli cells and the assembly of cell polarity complexes during spermatid differentiation. Also functions as an inhibitory somatodendritic cue that prevents the myelination of non-axonal parts of neurons. During myogenesis, it is involved in myocyte fusion. May also play a role in angiogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 393715113
• NCBI GeneID: 58494
• NCBI Accession #: NP_001257337.1
• NCBI GenBank Nucleotide #: NM_001270408.1
• UniProt Accession #: P57087; Q6UXG6; Q6YNC1; B2R6T9; B4DGT9
• Molecular Weight: 33,207 Da
• NCBI Official Full Name: junctional adhesion molecule B isoform 3
• NCBI Official Synonym Full Names: junctional adhesion molecule 2
• NCBI Official Symbol: JAM2
• NCBI Official Synonym Symbols: JAMB; CD322; JAM-B; VEJAM; PRO245; VE-JAM; C21orf43
• NCBI Protein Information: junctional adhesion molecule B; JAM-2; JAM-IT/VE-JAM; vascular endothelial junction-associated molecule
• UniProt Protein Name: Junctional adhesion molecule B
• UniProt Gene Name: JAM2
• UniProt Synonym Gene Names: C21orf43; VEJAM; JAM-B; JAM-2; VE-JAM
• UniProt Entry Name: JAM2_HUMAN

NCBI Description

This gene belongs to the immunoglobulin superfamily, and the junctional adhesion molecule (JAM) family. The protein encoded by this gene is a type I membrane protein that is localized at the tight junctions of both epithelial and endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types, and may play a role in lymphocyte homing to secondary lymphoid organs. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

JAM2: May play a role in the processes of lymphocyte homing to secondary lymphoid organs. Belongs to the immunoglobulin superfamily.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 21q21.2

Cellular Component: tight junction; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; protein heterodimerization activity

Biological Process: extracellular matrix organization and biogenesis; cell-cell adhesion; negative regulation of cell adhesion; blood coagulation; leukocyte migration