Rabbit anti-Human UBR7 Polyclonal Antibody | anti-UBR7 antibody

UBR7, NT (UBR7, C14orf130, Putative E3 ubiquitin-protein ligase UBR7, N-recognin-7) (Azide free) (HRP)

Cat. Num. AAA6360985

• Gene Names: UBR7; C14orf130
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: UBR7; Polyclonal Antibody; NT (UBR7; C14orf130; Putative E3 ubiquitin-protein ligase UBR7; N-recognin-7) (Azide free) (HRP); N-recognin-7; anti-UBR7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-UBR7 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: UBR7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-128 amino acids from the N-terminal region of human UBR7.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(UBR7 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the UBR7 antibody detected the UBR7 protein (arrow).)

Related Product Information for anti-UBR7 antibody

E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (By similarity).

Product Categories/Family for anti-UBR7 antibody

Antibodies; Ubiquitin Ligases

References

Lee, M.J., et al. Proc. Natl. Acad. Sci. U.S.A. 105(1):100-105(2008). Lim, J., et al. Cell 125(4):801-814(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 154426264
• NCBI GeneID: 55148
• UniProt Accession #: Q8N806
• NCBI Official Synonym Full Names: ubiquitin protein ligase E3 component n-recognin 7
• NCBI Official Symbol: UBR7
• NCBI Official Synonym Symbols: C14orf130
• NCBI Protein Information: putative E3 ubiquitin-protein ligase UBR7
• UniProt Protein Name: Putative E3 ubiquitin-protein ligase UBR7
• Protein Family: Putative E3 ubiquitin-protein ligase
• UniProt Gene Name: UBR7
• UniProt Synonym Gene Names: C14orf130
• UniProt Entry Name: UBR7_HUMAN

NCBI Description

This gene encodes a UBR box-containing protein that belongs to the E3 ubiquitin ligase family. The protein also contains a plant homeodomain (PHD) in the C-terminus. In mammals, the encoded protein recognizes N-degrons, the destabilizing N-terminal residues of short-lived proteins, which results in ubiquitinylation, and proteolysis via the proteasome. [provided by RefSeq, Jul 2016]