Rabbit anti-Human UBP46 Polyclonal Antibody | anti-UBP46 antibody

UBP46, CT (USP46, Ubiquitin carboxyl-terminal hydrolase 46, Deubiquitinating enzyme 46, Ubiquitin thioesterase 46, Ubiquitin-specific-processing protease 46) (APC)

Cat. Num. AAA6360960

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: UBP46; Polyclonal Antibody; CT (USP46; Ubiquitin carboxyl-terminal hydrolase 46; Deubiquitinating enzyme 46; Ubiquitin thioesterase 46; Ubiquitin-specific-processing protease 46) (APC); USP46; Ubiquitin-specific-processing protease 46; anti-UBP46 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-UBP46 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: UBP46 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 240-269 amino acids from the C-terminal region of human UBP46.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(UBP46 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the UBP46 antibody detected the UBP46 protein (arrow).)

Related Product Information for anti-UBP46 antibody

Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP46 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes (Quesada et al., 2004 [PubMed 14715245]).

Product Categories/Family for anti-UBP46 antibody

Antibodies; Ubiquitin

References

Kushima, I., et al. J. Hum. Genet. 55(3):133-136(2010). Cohn, M.A., et al. J. Biol. Chem. 284(8):5343-5351(2009). Zemunik, T., et al. Croat. Med. J. 50(1):23-33(2009). Quesada, V., et al. Biochem. Biophys. Res. Commun. 314(1):54-62(2004). Puente, X.S., et al. Nat. Rev. Genet. 4(7):544-558(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 197116355
• NCBI GeneID: 64854
• NCBI Accession #: NP_001127695.1; NP_073743.2
• NCBI GenBank Nucleotide #: NM_001134223.1
• UniProt Accession #: P62068; Q80V95; Q9H7U4; Q9H9T8; B7Z3Y7; B7Z675; B7Z7S3; G8ACC7
• Molecular Weight: 41,112 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 46 isoform 2
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 46
• NCBI Official Symbol: USP46
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 46; deubiquitinating enzyme 46; ubiquitin thioesterase 46; ubiquitin thiolesterase 46; ubiquitin-specific-processing protease 46
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 46
• UniProt Gene Name: USP46
• UniProt Entry Name: UBP46_HUMAN

NCBI Description

Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP46 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes (Quesada et al., 2004 [PubMed 14715245]).[supplied by OMIM, Jun 2009]

Uniprot Description

USP46: Deubiquitinating enzyme that plays a role in behavior, possibly by regulating GABA action. May act by mediating the deubiquitination of GAD1/GAD67. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. Not involved in deubiquitination of monoubiquitinated FANCD2. Belongs to the peptidase C19 family. USP12/USP46 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin-specific protease; Protease; EC 3.4.19.12

Chromosomal Location of Human Ortholog: 4q12

Molecular Function: protein binding; ubiquitin-specific protease activity

Biological Process: ubiquitin-dependent protein catabolic process; behavioral fear response; protein deubiquitination; righting reflex; adult feeding behavior; behavioral response to ethanol; regulation of synaptic transmission, GABAergic