Rabbit anti-Human UBP1 Polyclonal Antibody | anti-UBP1 antibody

UBP1, ID (UBP1, LBP1, Upstream-binding protein 1, Transcription factor LBP-1) (Azide free) (HRP)

Cat. Num. AAA6360930

• Gene Names: UBP1; LBP1A; LBP1B; LBP-1B; LBP-1a
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: UBP1; Polyclonal Antibody; ID (UBP1; LBP1; Upstream-binding protein 1; Transcription factor LBP-1) (Azide free) (HRP); Transcription factor LBP-1; anti-UBP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human UBP1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-UBP1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 266-295 in the c-terminal region of human UBP1, UniProt Accession #Q9NZ17. Species sequence homology: mouse
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis using MBS6007396 in HepG2 cell line lysates (35ug/lane).T his demonstrates the UBP1 antibody detected the UBP1 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using MBS6007396 in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Immunofluorescence (IF)

(Fluorescent image of A549 cell stained withMBS6007396. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with UBP1 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).UBP1 immunoreactivity is localized to Nucleus significantly.)

Related Product Information for anti-UBP1 antibody

UBP1 functions as a transcriptional activator in a promoter context-dependent manner. Modulates the placental expression of CYP11A1. Involved in regulation of the alpha-globin gene in erythroid cells. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with TFCP2 (By similarity). Involved in regulation of the alpha-globin gene in erythroid cells. Binds strongly to sequences around the HIV-1 initiation site and weakly over the TATA-box. Represses HIV-1 transcription by inhibiting the binding of TFIID to the TATA-box.

Product Categories/Family for anti-UBP1 antibody

Antibodies; Transcription Factors

References

1. Katsura, A., et al. Genes Cells 14(10):1183-1196(2009). 2. Koutnikova, H., et al. PLoS Genet. 5 (8), E1000591 (2009). 3. Henderson, Y.C., et al. DNA Cell Biol. 27(2):71-79(2008). 4. Huang, N., et al. Mol. Endocrinol. 19(2):409-420(2005). 5. Huang, N., et al. J. Biol. Chem. 275(4):2852-2858(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 189491632
• NCBI GeneID: 7342
• NCBI Accession #: NP_001121632.1; NP_055332.3; NP_001121633.1
• NCBI GenBank Nucleotide #: NM_001128160.1
• UniProt Accession #: Q9NZI7; Q68CT0; Q86Y57; Q9H8V0; Q9UD76; Q9UD78
• Molecular Weight: 56,453 Da
• NCBI Official Full Name: upstream-binding protein 1 isoform LBP-1a
• NCBI Official Synonym Full Names: upstream binding protein 1 (LBP-1a)
• NCBI Official Symbol: UBP1
• NCBI Official Synonym Symbols: LBP1A; LBP1B; LBP-1B; LBP-1a
• NCBI Protein Information: upstream-binding protein 1
• UniProt Protein Name: Upstream-binding protein 1
• Protein Family: UBP1-associated protein
• UniProt Gene Name: UBP1
• UniProt Synonym Gene Names: LBP1
• UniProt Entry Name: UBIP1_HUMAN

Uniprot Description

UBP1: Functions as a transcriptional activator in a promoter context-dependent manner. Modulates the placental expression of CYP11A1. Involved in regulation of the alpha-globin gene in erythroid cells. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with TFCP2. Involved in regulation of the alpha-globin gene in erythroid cells. Binds strongly to sequences around the HIV-1 initiation site and weakly over the TATA-box. Represses HIV-1 transcription by inhibiting the binding of TFIID to the TATA-box. Belongs to the grh/CP2 family. CP2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 3p22.3

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: DNA binding; sequence-specific DNA binding; transcription factor activity; transcription corepressor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; viral genome replication; angiogenesis; negative regulation of transcription, DNA-dependent