Western Blot (WB)
(UBFD1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the UBFD1 antibody detected the UBFD1 protein (arrow).)
Rabbit anti-Human UBFD1 Polyclonal Antibody | anti-UBFD1 antibody
UBFD1, NT (UBFD1, UBPH, Ubiquitin domain-containing protein UBFD1, Ubiquitin-binding protein homolog)
Gene Names
UBFD1; UBPH
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
UBFD1; Polyclonal Antibody; NT (UBFD1; UBPH; Ubiquitin domain-containing protein UBFD1; Ubiquitin-binding protein homolog); Anti -UBFD1; anti-UBFD1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-UBFD1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Immunogen
UBFD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human UBFD1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(UBFD1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the UBFD1 antibody detected the UBFD1 protein (arrow).)
Western Blot (WB)
(UBFD1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the UBFD1 antibody detected the UBFD1 protein (arrow).)
Immunohistochemistry (IHC)
(UBFD1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UBFD1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(UBFD1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UBFD1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(UBFD1 Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(UBFD1 Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-UBFD1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
33,382 Da
NCBI Official Full Name
Ubiquitin domain-containing protein UBFD1
NCBI Official Synonym Full Names
ubiquitin family domain containing 1
NCBI Official Symbol
UBFD1
NCBI Official Synonym Symbols
UBPH
NCBI Protein Information
ubiquitin domain-containing protein UBFD1; ubiquitin-binding protein homolog
UniProt Protein Name
Ubiquitin domain-containing protein UBFD1
Protein Family
UniProt Gene Name
UBFD1
UniProt Synonym Gene Names
UBPH
UniProt Entry Name
UBFD1_HUMAN
Uniprot Description
UBFD1: May play a role as NF-kappa-B regulator.
Chromosomal Location of Human Ortholog: 16p12
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Product Notes
The UBFD1 ubfd1 (Catalog #AAA6000126) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UBFD1, NT (UBFD1, UBPH, Ubiquitin domain-containing protein UBFD1, Ubiquitin-binding protein homolog) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's UBFD1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the UBFD1 ubfd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBFD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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