Rabbit IKBKG Polyclonal Antibody | anti-IKBKG antibody

IKBKG Antibody

Cat. Num. AAA9401790

• Gene Names: IKBKG; IP; IP1; IP2; FIP3; IPD2; NEMO; FIP-3; Fip3p; IMD33; AMCBX1; ZC2HC9; IKK-gamma
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: IKBKG; Polyclonal Antibody; IKBKG Antibody; AMCBX1; FIP-3; FIP3; Fip3p; anti-IKBKG antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total IKBKG protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 487

• Applicable Applications for anti-IKBKG antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human IKBKG.
• Target Name: IKBKG
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using IKBKG antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat testis using IKBKG antibody at dilution of 1:500 (400x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF7 cell using IKBKG antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-IKBKG antibody

The NF-kB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkB proteins (1-3). Most agents that activate NF-kB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IkB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IkB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKa and IKKb serve as the catalytic subunits of the kinase and IKKg serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation of Ser177 and Ser181 in the activation loop of IKKb (Ser176 and Ser180 in IKKa), which causes conformational changes resulting in kinase activation (10-13).

Product Categories/Family for anti-IKBKG antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 223671870
• NCBI GeneID: 8517
• NCBI Accession #: NP_001093326.2
• NCBI GenBank Nucleotide #: NM_001099856.3
• UniProt Accession #: Q9Y6K9; Q7LBY6; Q7Z7F1
• Molecular Weight: 36,953 Da
• NCBI Official Full Name: NF-kappa-B essential modulator isoform b
• NCBI Official Synonym Full Names: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
• NCBI Official Symbol: IKBKG
• NCBI Official Synonym Symbols: IP; IP1; IP2; FIP3; IPD2; NEMO; FIP-3; Fip3p; IMD33; AMCBX1; ZC2HC9; IKK-gamma
• NCBI Protein Information: NF-kappa-B essential modulator
• UniProt Protein Name: NF-kappa-B essential modulator
• UniProt Gene Name: IKBKG
• UniProt Synonym Gene Names: FIP3; NEMO; NEMO; IKKAP1; I-kappa-B kinase subunit gamma; IKK-gamma; IKKG; IkB kinase subunit gamma
• UniProt Entry Name: NEMO_HUMAN

NCBI Description

This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome. [provided by RefSeq, Aug 2011]

Uniprot Description

IKKG: a regulatory subunit of the IKK-signalosome complex. Interacts preferentially with IKK-beta but also able to interact with IKK-alpha, IKAP, TAX, RIP and MAP3K14/NIK. Defects are the cause of familial incontinentia pigmenti type II (IP2).

Protein type: Adaptor/scaffold; Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: spindle pole; cytoplasm; intracellular; IkappaB kinase complex; cytosol; nucleus; ubiquitin ligase complex

Molecular Function: protein domain specific binding; protein binding; signal transducer activity; peroxisome proliferator activated receptor binding; protein homodimerization activity; protein heterodimerization activity; metal ion binding; ubiquitin protein ligase binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; establishment of vesicle localization; viral reproduction; activation of MAPK activity; apoptosis; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; toll-like receptor 10 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 5 signaling pathway; B cell homeostasis; positive regulation of interferon type I production; JNK cascade; inflammatory response; toll-like receptor 4 signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; MyD88-independent toll-like receptor signaling pathway; response to virus; activation of NF-kappaB-inducing kinase; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; immune response; toll-like receptor 9 signaling pathway; response to DNA damage stimulus

Disease: Invasive Pneumococcal Disease, Recurrent Isolated, 2; Ectodermal Dysplasia, Hypohidrotic, With Immune Deficiency; Immunodeficiency Without Anhidrotic Ectodermal Dysplasia; Immunodeficiency 33; Incontinentia Pigmenti; Ectodermal Dysplasia, Anhidrotic, With Immunodeficiency, Osteopetrosis, And Lymphedema