Western Blot (WB)
(Western Blot detection against Immunogen (34.25kD).)
Mouse anti-Human ATG12 Monoclonal Antibody | anti-ATG12 antibody
ATG12 (Ubiquitin-like Protein ATG12, Autophagy-related Protein 12, APG12-like, APG12, APG12L) (HRP)
Gene Names
ATG12; APG12; FBR93; APG12L; HAPG12
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ATG12; Monoclonal Antibody; ATG12 (Ubiquitin-like Protein ATG12; Autophagy-related Protein 12; APG12-like; APG12; APG12L) (HRP); anti-ATG12 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
2H8
Specificity
Recognizes human ATG12.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Applicable Applications for anti-ATG12 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length recombinant corresponding to aa1-75 from ATG12 (AAH11033) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MAEEPQSVLQLPTSIAAGGEGLTDVSPETTTPEPPSSAAVSPGTEEPAGDTKKKIYLCESVLCSFPRPRSWNSL
Conjugate
HRP
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot detection against Immunogen (34.25kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (34.25kD).)
Testing Data
(Detection limit for recombinant GST tagged ATG12 is ~1ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged ATG12 is ~1ng/ml as a capture antibody.)
Related Product Information for anti-ATG12 antibody
Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5.
Product Categories/Family for anti-ATG12 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
7,746 Da
NCBI Official Full Name
Homo sapiens ATG12 autophagy related 12 homolog (S. cerevisiae), mRNA
NCBI Official Synonym Full Names
autophagy related 12
NCBI Official Symbol
ATG12
NCBI Official Synonym Symbols
APG12; FBR93; APG12L; HAPG12
NCBI Protein Information
ubiquitin-like protein ATG12
Protein Family
NCBI Description
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008]
Research Articles on ATG12
Similar Products
Product Notes
The ATG12 (Catalog #AAA6151325) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ATG12 (Ubiquitin-like Protein ATG12, Autophagy-related Protein 12, APG12-like, APG12, APG12L) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ATG12 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ATG12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATG12, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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