Rabbit anti-Human UBE3A Polyclonal Antibody | anti-UBE3A antibody

UBE3A, CT (Ubiquitin-protein Ligase E3A, E6AP Ubiquitin-protein Ligase, Oncogenic Protein-associated Protein E6-AP, Human Papillomavirus E6-associated Protein, Renal Carcinoma Antigen NY-REN-54, E6AP, EPVE6AP, HPVE6A)

Cat. Num. AAA627491

• Gene Names: UBE3A; AS; ANCR; E6-AP; HPVE6A; EPVE6AP; FLJ26981
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: UBE3A; Polyclonal Antibody; CT (Ubiquitin-protein Ligase E3A; E6AP Ubiquitin-protein Ligase; Oncogenic Protein-associated Protein E6-AP; Human Papillomavirus E6-associated Protein; Renal Carcinoma Antigen NY-REN-54; E6AP; EPVE6AP; HPVE6A); Anti -UBE3A; anti-UBE3A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human UBE3A.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-UBE3A antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:1000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa843-873 from the C-terminal region of human UBE3A.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of UBE3A Antibody (C-term) in T47D cell line lysates (35ug/lane).UBE3A(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of UBE3A Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(UBE3A Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-UBE3A antibody

UBE3A interacts with the E6 protein of the cancer-associated human papillomavirus types 16 and 18. The E6/E6-AP complex binds to and targets the p53 tumor-suppressor protein for ubiquitin-mediated proteolysis. It is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It can target itself for ubiquitination in vitro and efficiently promotes its own degradation in vivo. It appears that only unmodified E6-AP molecules can bind efficiently to p53 in the presence of the HPV E6 oncoprotein. UBE3A binds UBQLN1 and UBQLN2. Defects in UBE3A are a cause of Angelman syndrome (AS) [MIM:105830]; also known as 'happy puppet syndrome'. AS is characterized by features of severe motor and intellectual retardation, microcephaly, ataxia, frequent jerky limb movements and flapping of the arms and hands, hypotonia, hyperactivity, hypopigmentation, seizures, absence of speech, frequent smiling and episodes of paroxysmal laughter, and an unusual facies characterized by macrostomia, a large mandible and open-mouthed expression, a great propensity for protruding the tongue ('tongue thrusting'), and an occipital groove. UBE3A contains 1 HECT-type E3 ubiquitin-protein ligase domain.

Product Categories/Family for anti-UBE3A antibody

Antibodies; Abs to Ubiquitin Ligases

NCBI and Uniprot Product Information

• NCBI GI #: 19718766
• NCBI GeneID: 7337
• NCBI Accession #: NP_000453.2
• NCBI GenBank Nucleotide #: NM_000462.3
• UniProt Accession #: Q05086; P78355; Q93066; Q9UEP4; Q9UEP5; Q9UEP6; Q9UEP7; Q9UEP8; Q9UEP9; A8K8Z9; Q5W7F7; Q5W7F8; Q96GR7; Q9BUI6; Q9H2G0; Q9UBN9
• Molecular Weight: 100,688 Da
• NCBI Official Full Name: ubiquitin-protein ligase E3A isoform 2
• NCBI Official Synonym Full Names: ubiquitin protein ligase E3A
• NCBI Official Symbol: UBE3A
• NCBI Official Synonym Symbols: AS; ANCR; E6-AP; HPVE6A; EPVE6AP; FLJ26981
• NCBI Protein Information: ubiquitin-protein ligase E3A; CTCL tumor antigen se37-2; E6AP ubiquitin-protein ligase; renal carcinoma antigen NY-REN-54; human papillomavirus E6-associated protein; oncogenic protein-associated protein E6-AP; human papilloma virus E6-associated protein
• UniProt Protein Name: Ubiquitin-protein ligase E3A
• Protein Family: Ubiquitin-protein ligase
• UniProt Gene Name: UBE3A
• UniProt Synonym Gene Names: E6AP; EPVE6AP; HPVE6A
• UniProt Entry Name: UBE3A_HUMAN

NCBI Description

This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined. [provided by RefSeq]

Uniprot Description

UBE3A: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator p27Kip1. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo. Binds UBQLN1 and UBQLN2. Interacts with the 26S proteasome. Interacts with HCV core protein and targets it to degradation. Interacts with the E6 protein of the cancer- associated human papillomavirus types 16 and 18. The E6/E6-AP complex binds to and targets the p53/TP53 tumor-suppressor protein for ubiquitin-mediated proteolysis. Interacts with BPY2. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; Nuclear receptor co-regulator; Ubiquitin ligase; Transcription, coactivator/corepressor; EC 6.3.2.-; EC 6.3.2.19; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 15q11.2

Cellular Component: proteasome complex; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; transcription coactivator activity; ubiquitin-protein ligase activity; ligase activity

Biological Process: positive regulation of phosphoinositide 3-kinase cascade; ubiquitin-dependent protein catabolic process; protein autoubiquitination; sperm entry; ovarian follicle development; viral reproduction; androgen receptor signaling pathway; protein ubiquitination during ubiquitin-dependent protein catabolic process; positive regulation of transcription from RNA polymerase II promoter; brain development; regulation of circadian rhythm; proteolysis

Disease: Angelman Syndrome