Rabbit anti-Human, Mouse UBE2V1 Polyclonal Antibody | anti-UBE2V1 antibody

UBE2V1, CT (Ubiquitin-conjugating Enzyme E2 Variant 1, UEV-1, CROC-1, TRAF6-Regulated IKK Activator 1 beta Uev1A, CROC1, UBE2V, UEV1, P/OKcl.19) (Azide free) (HRP)

Cat. Num. AAA6504742

• Gene Names: UBE2V1; CIR1; UEV1; CROC1; UBE2V; UEV-1; UEV1A; CROC-1
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: UBE2V1; Polyclonal Antibody; CT (Ubiquitin-conjugating Enzyme E2 Variant 1; UEV-1; CROC-1; TRAF6-Regulated IKK Activator 1 beta Uev1A; CROC1; UBE2V; UEV1; P/OKcl.19) (Azide free) (HRP); anti-UBE2V1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human UBE2V1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-UBE2V1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human UBE2V1 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of UBE2V1 Antibody (C-term) in mouse cerebellum tissue lysates (35ug/lane). UBE2V1 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of UBE2V1 (arrow) using rabbit polyclonal UBE2V1 Antibody (M151). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2V1 gene.)

Product Categories/Family for anti-UBE2V1 antibody

Antibodies; Ubiquitin

References

1. Thomson, T.M., et al., Genome Res. 10(11):1743-1756 (2000). 2. Long, M., Genome Res. 10(11):1655-1657 (2000). 3. Deng, L., et al., Cell 103(2):351-361 (2000). 4. Hofmann, R.M., et al., Cell 96(5):645-653 (1999). 5. Ma, L., et al., Oncogene 17(10):1321-1326 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 40806164
• NCBI GeneID: 7335
• NCBI Accession #: NP_068823
• NCBI GenBank Nucleotide #: NM_021988
• UniProt Accession #: Q9GZW1; Q13404
• Molecular Weight: 19kDa
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 variant 1 isoform a
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 V1
• NCBI Official Symbol: UBE2V1
• NCBI Official Synonym Symbols: CIR1; UEV1; CROC1; UBE2V; UEV-1; UEV1A; CROC-1
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 variant 1

NCBI Description

Ubiquitin-conjugating E2 enzyme variant proteins constitute a distinct subfamily within the E2 protein family. They have sequence similarity to other ubiquitin-conjugating enzymes but lack the conserved cysteine residue that is critical for the catalytic activity of E2s. The protein encoded by this gene is located in the nucleus and can cause transcriptional activation of the human FOS proto-oncogene. It is thought to be involved in the control of differentiation by altering cell cycle behavior. Alternatively spliced transcript variants encoding multiple isoforms have been described for this gene, and multiple pseudogenes of this gene have been identified. Co-transcription of this gene and the neighboring upstream gene generates a rare transcript (Kua-UEV), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Apr 2012]