Goat UBE2I Polyclonal Antibody | anti-UBE2I antibody

UBE2I (UBE2I, ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast), C358B7.1, P18, UBC9, SUMO-1-protein ligase, ubiquitin carrier protein, ubiquitin conjugating enzyme 9, ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9), ubiquitin-conjugating

Cat. Num. AAA614179

• Gene Names: UBE2I; P18; UBC9; C358B7.1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: UBE2I; Polyclonal Antibody; UBE2I (UBE2I; ubiquitin-conjugating enzyme E2I (UBC9 homolog; yeast); C358B7.1; P18; UBC9; SUMO-1-protein ligase; ubiquitin carrier protein; ubiquitin conjugating enzyme 9; ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9); ubiquitin-conjugating; Anti -UBE2I (UBE2I; anti-UBE2I antibody

• Host: Goat
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: This antibody is expected to recognize all reported isoforms (NP_003336.1, NP_919235.1, NP_919236.1 and NP_919237.1). Species Crossreactivity: human, mouse, rat, chicken hamster, Xenopus.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-UBE2I antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Peptide ELISA:1:64,000; Western Blot: 0.1-0.3ug/ml. ~17kD band observed in Human kidney, lung and testis lysates; Immunohistochemistry: 1ug/ml shows nuclear staining of some epithelial cells of glomeruli and renal tubules in paraffin embedded Human Kidney.
• Immunogen: SGIALSRLAQERK-C, N Terminus
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-UBE2I antibody

Antibodies; Abs to Ubiquitin Ligases

NCBI and Uniprot Product Information

• NCBI GI #: 5262325
• NCBI GeneID: 7329
• UniProt Accession #: P63279; P50550; Q15698; Q59GX1; Q86VB3; D3DU69; Q7KZS0
• Molecular Weight: 18,007 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2I
• NCBI Official Symbol: UBE2I
• NCBI Official Synonym Symbols: P18; UBC9; C358B7.1
• NCBI Protein Information: SUMO-conjugating enzyme UBC9; OTTHUMP00000045512; OTTHUMP00000158627; OTTHUMP00000158628; OTTHUMP00000158629; OTTHUMP00000198126; OTTHUMP00000198127; OTTHUMP00000198131; SUMO-protein ligase; SUMO-1-protein ligase; ubiquitin-protein ligase I; ubiquitin carrier protein 9; ubiquitin carrier protein I; ubiquitin-protein ligase E2I; ubiquitin conjugating enzyme 9; ubiquitin-conjugating enzyme UbcE2A; ubiquitin-like protein SUMO-1 conjugating enzyme; ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast); ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9)
• UniProt Protein Name: SUMO-conjugating enzyme UBC9
• UniProt Gene Name: UBE2I
• UniProt Synonym Gene Names: UBC9; UBCE9
• UniProt Entry Name: UBC9_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Uniprot Description

UBC9: Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly- SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Interacts with HIPK1, HIPK2, PPM1J, RASD2 and TCF3 Interacts with NR2C1; the interaction promotes its sumoylation. Forms a tight complex with RANGAP1 and RANBP2. Interacts with SIAH1 and PARP. Interacts with various transcription factors such as TFAP2A, TFAP2B, TFAP2C, AR, ETS1 and SOX4. Interacts with RWDD3; the interaction enhances the sumoylation of a number of proteins such as HIF1A and I-kappa-B. Interacts with DNMT1. Interacts with FOXL2. Forms a complex with SENP6 and UBE2I in response to UV irradiation. Interacts with human herpesvirus 6 IE2. Interacts with human adenovirus early E1A protein; this interaction interferes with polysumoylation (Probable). Interacts with DNM1l (via its GTPase and B domains); the interaction promotes sumoylation of DNM1L, mainly in its B domain. Interacts with PML-RARA oncoprotein (via the coiled-colied domain); the interaction is required for sumoylation of the PML- RARA oncoprotein. Interacts with IPO13. Interacts with NFATC2IP; this inhibits formation of poly-SUMO chains. Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: Ligase; Ubiquitin ligase; Ubiquitin conjugating system; SUMO conjugating system; Nuclear receptor co-regulator; EC 6.3.2.-; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; PML body; cytoplasm; dendrite; fibrillar center; synapse; synaptonemal complex; nucleus

Molecular Function: protein C-terminus binding; protein binding; enzyme binding; ubiquitin protein ligase binding; bHLH transcription factor binding; HLH domain binding; transcription factor binding; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; mitosis; viral reproduction; positive regulation of steroid hormone receptor signaling pathway; protein ubiquitination; protein modification process; negative regulation of transcription from RNA polymerase II promoter; post-translational protein modification; chromosome segregation; cellular protein metabolic process; protein sumoylation; cell division; positive regulation of transcription factor activity; regulation of receptor activity; negative regulation of transcription, DNA-dependent